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What is NANO?. 1 nanometer (nm) = 10 -9 meters (m) ONE BILLIONTH OF A METER!. Atom: ~0.1 nm DNA double helix: ~2 nm wide Protein: ~10 nm long Bacteria: 200 nm Human hair: 100,000 nm wide Paper: 100,000 nm thick Girl 4 feet tall: ~1.2 × 10 9 nm tall
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What is NANO? 1 nanometer (nm) = 10-9 meters (m) ONE BILLIONTH OF A METER! Atom: ~0.1 nm DNA double helix: ~2 nm wide Protein: ~10 nm long Bacteria: 200 nm Human hair: 100,000 nm wide Paper: 100,000 nm thick Girl 4 feet tall: ~1.2 × 109 nm tall Empire State Building: ~3.81 × 1011 nm tall
How do we ‘see’ NANO? The smallest object that our eyes can see is the width of a human hair. That’s 100,000 nm! NANOis sosmallthat we need special instrumentsto ‘see’!
‘See’ by feeling ~30 mm scan 1 nm = 10-9 m mm = micrometer 1 mm = 10-6 m http://www.nanotech-now.com/Art_Gallery/antonio-siber.htm
Basic operation of the AFM Measures attractive or repulsive forces between a tip and the sample by using a laser to detect lever motion
AFM Tips http://www.tedpella.com/probes_html/budgetsensors.htm
AFM Tips The tip is roughly 20 µm long, the cantilever is 450 µm in length and 20-50 µm wide, and the thickness is usually 3-4 µm thick 20 mm 450 mm mm = micrometer 1 mm = 10-6 m http://www.tedpella.com/probes_html/budgetsensors.htm
Contact Mode To get an image, a sharp tip is scanned across a surface
Tip Size Limitations Z Resolutions: X and Y: 2 – 10 nm Z: 0.05 nm
DNA Origami http://www.molbiotech.uni-freiburg.de/km/KM_main.htm
DNA Origami Nature, 2006, 440, 297-302.
Human chromosome CD Polymer stamp