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96 Aa

Crude one-pot assembly. Reduced N protein. PDB : 1BHT, Ultsch, M. Structure 1998 , 6 , 1383-1393. Folding 1 h. Folding 24 h. One-pot total synthesis of N HGF domain. S. S. a chain. b chain. Hepatocyte growth factor 90 kD. N. K1. K2. K3. K4. HPLC purified.

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96 Aa

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  1. Crude one-pot assembly Reduced N protein PDB : 1BHT, Ultsch, M. Structure1998, 6, 1383-1393 Folding 1 h Folding 24 h One-pot total synthesis of N HGF domain S S a chain b chain Hepatocyte growth factor 90 kD N K1 K2 K3 K4 HPLC purified • Disulfide bonds • Functional • Binding to heparansulfate, Heparin 96 Aa 2 disulfide bridges

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