1 / 25

Molecular Spectrometry (UV and Visible) Part 1: Absorption

Molecular Spectrometry (UV and Visible) Part 1: Absorption. Instrument Evolution. Beckman DU. Replaced prism with grating. Turner Spectrometer. Spec 20 as well. Cary and Perkin-Elmer. Dual Beam High resolution. PDA Hewlett Packard. Fiber Optic Probes Ocean Optics.

dahlia
Download Presentation

Molecular Spectrometry (UV and Visible) Part 1: Absorption

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Molecular Spectrometry (UV and Visible)Part 1: Absorption

  2. Instrument Evolution Beckman DU

  3. Replaced prism with grating

  4. Turner Spectrometer Spec 20 as well

  5. Cary and Perkin-Elmer Dual Beam High resolution

  6. PDA Hewlett Packard

  7. Fiber Optic Probes Ocean Optics

  8. Signal Expressions Eout = Φλ (Ω/4π) Tm Ts Rλ G Φλ= source spectral power (W/nm) Tm = monochromator throughput (nm) Ts = Sample transmittance Rλ= Detector Responsivity (A/W) G = Gain of electronics (V/A)

  9. Signal Expressions Tm = (WH/Aeff) Δλeff Top W= slit width (cm) H= slit height (cm) Aeff = Effective area of source image (cm2) Δλeff= Effective bandwitdh of the monochromator (nm) Top = Transmittance of optics

  10. Signal Expressions Ts = e-εbc = Esam/Eref Eref = Output voltage for a blank Esam = Output voltage for sample

  11. Signal Expressions A = -log (Esam/Eref) = -log (Ts) = ε b c Notes: 1. Since UV-Vis absorbance bands are much wider than the mono- chromator bandwidth, Beer’s Law assumes that A does not depend on Δλeff. 2. ε is wavelength dependent, but does not depend on any other instrumental parameter.

  12. A = -log (Esam/Eref) = -log (Ts) = ε b c

  13. A = ε b c Design Cell to Maximize b

  14. Deviations from Beer’s Law 1. Optical a) slit width b) wavelength selection c) stray radiation 2. Chemical a) high analyte concentration b) pH dependence c) mixtures

  15. Deviations from Beer’s Law Slit Width

  16. Deviations from Beer’s Law

  17. Deviations from Beer’s Law Wavelength Selection

  18. Deviations from Beer’s Law Stray Radiation Effect No Stray Light 10% Stray Light

  19. Deviations from Beer’s Law High Analyte Concentrations

  20. Deviations from Beer’s Law pH Dependence

  21. Deviations from Beer’s Law Mixtures: Absorbance is Additive

  22. Deviations from Beer’s Law Mixtures: Solve multiple equations!

  23. Deviations from Beer’s Law Mixtures: When all else fails, Separate the mixture components

  24. UV Absorbance Detector for HPLC

  25. Final Note on UV-Vis Signals The Absorbance (Analytical Signal) does not depend on the magnitude of the source signal (except for the deviations discussed above). The Noise in the measurement often DOES depend on the source signal level.

More Related