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200µm

a. c. d. 200µm. 200µm. b. e. f. 30µm. Supplemental Data Figure 2. b.

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200µm

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  1. a c d 200µm 200µm b e f 30µm Supplemental Data Figure 2 b Staining for acLDL (a-c) and CRP (d-f) in HUVECs after preincubation with 0.1% FCS (a, d), 10 mg/L nCRP (b, e) and 10 mg/L mCRP (c, f). White arrows in a and b indicate LDL containing vesicles which were reduced in mCRP-treated cells (c). Blue colour indicates staining for nuclear DNA (DAPI, d-f), green colour indicates nCRP (e) and mCRP staining (d, f). Cells treated with nCRP (10 mg/L) were nCRP negative (e). In cells incubated with mCRP (10 mg/L, f), predominant membrane staining was observed (white arrow), while control cells (d) showed no mCRP-staining.

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