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WSSP Restriction Digest Gels

WSSP Restriction Digest Gels. October 2010. 17.AXB02.10. 17.SAT01.10. 17.AJK01.10. 17.LNU02.10. U. C. C. U. C. U. U. Section 2/7 Restriction digest Gel1. 2.9. 2.9. 2.9. 17.GCP01.10. 17.LNU02.10. 17.DP01.10. 17.CJM01.10. U. C. C. C. U. U. C. 2.9. 2.9. 2.9. 2.9.

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WSSP Restriction Digest Gels

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  1. WSSP Restriction Digest Gels October 2010

  2. 17.AXB02.10 17.SAT01.10 17.AJK01.10 17.LNU02.10 U C C U C U U Section 2/7 Restriction digest Gel1 2.9 2.9 2.9 17.GCP01.10 17.LNU02.10 17.DP01.10 17.CJM01.10 U C C C U U C 2.9 2.9 2.9 2.9

  3. Section 3-4 Gel 1 Sakai gel 2 17.MEK01.10 17.DJA01.10 17.MCB02.10 17.EJF ??.10 U U C U C C U 2.9 2.9 No result, PCR: 600 Insert 1100 No PCR result Insert 1200 No PCR result 17.MEK01.10 17.JWG01.10 17.BAH.10 17.????.10 U C C C U U C 2.9?? 2.9 2.9 Not sure about 2.9 Band in digest lane Are the other bands Internal cuts sites No PCR result to confirm Unsure about Insert, which band? No result

  4. Section 34 gel 2 SAKAI GEL 3 C U C U C U 2.9 2.9 2.9 What is the bright band at 2KB? Internal cut site? Star activity? PCR result: Internal cut site? Star activity? PCR result: 17.SW01.10 C U C U C U 2.9 2.9 2.9 Insert : 1200 PCR result: What is the bright band at 2KB? Internal cut site? Star activity? PCR result: ?? What is the bright band at 2KB? Internal cut site? Star activity? PCR result: 1350

  5. 17.HMB01.10 17.NB01.10 17.MKB01.10 17.YC01.10 Section 56 gel 1 SAKAI GEL 4 U C U C C U 2.9 2.9 2.9 Are these the inserts? Are the other bands star activity? PCR result: 1,000 Why is the uncut lane running so high? Reversed tubes? 17.LGG01.10 17.AF02.10 17.YC01.10 17.AF01.10 C U C U C U C 2.9 Reversed tubes? No DNA Insert :1000 No PCR run No digest PCR result: 475

  6. Section 56 gel 2 SAKAI GEL 5 17.AJF03.10 17.KET02.10 17.JAT02.10 2.9 2.9 2.9 Insert :1050 Star Activity? Large insert? Insert too small

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