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J.T. Rosenberg, K. Sellgren , F. Calixto Bejarano ,

Tracking of normoxic and hypoxic pretreated adult human m esenchymal s tem cells in association with stroke utilizing 1 H and 23 Na at ultra-high magnet field MRI. J.T. Rosenberg, K. Sellgren , F. Calixto Bejarano , M . Baird, M.W. Davidson, T. Ma & S.C. Grant

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J.T. Rosenberg, K. Sellgren , F. Calixto Bejarano ,

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  1. Tracking of normoxic and hypoxic pretreated adult human mesenchymal stem cells in association with stroke utilizing 1H and 23Na at ultra-high magnet field MRI J.T. Rosenberg, K. Sellgren, F. CalixtoBejarano, M. Baird, M.W. Davidson, T. Ma & S.C. Grant NHMFL and The Florida State University Cell therapy is being evaluated as a potential treatment for ischemic stroke. Hypoxic pretreated hMSCs have show increased resistant to ischemic stroke environment and added therapeutic properties. In this in vivo study,normoxic and hypoxic cultured hMSCs were labeled with a fluorescent micron sized iron oxides (MPIOs) and injected intra-arterially to treat ischemic stroke resulting from a middle cerebral artery occlusion (MCAO). Serial MRI at 21.1 T was performed to acquire 1H and 23Na images of lesion progression, cell migration & longitudinal contrast. Methods Results • MR equipment for in vivoexperiments: • 21.1-T UWB vertical magnet • Double tuned 1H and 23Na 33-mm RF birdcage coil • in vivo imaging protocols: • Stroke assessment:2D T2 weighted Spin echo (SE) • Labeled cells:3D gradient recalled echo (GRE) • Sodium in stroke:3D 23Na GRE • Middle cerebral artery occlusion (MCAO): • -- following Longa et al (1989) and Uluçet al(2011) • Male Sprague Dawley rats weighing • 1-hr occlusion following re-perfusion • Intra-arterial injection of hMSCs: • 1 millions cells labeled with MPIO into intracarotidartery • MRI at 24 and 1 week following surgery Percent decrease in stroke lesion area for 1H and 23Na images T2- weighted image 23Na image Segmented stroke lesion (yellow) Segmented stroke lesion (purple) 23Na onlyshows significant difference in stroke recovery • Conclusions: • Significant clearance of hSMCs with in 1 week • T2W and 23Na images show lesion size recovery • High field 23Na imaging appears to be a more sensitive indicator of physiological effects of MSC therapy on stroke lesion recovery Percent decrease: 47.8 % 74.2 % Facilities:900-MHz UWBNMR Magnet, Tallahassee, FL Citation: Rosenberg, J.T.,Sellgren, K., Bejarano, F.C., Baird, M., Davidosn, M., Teng, M., Grant, S.C., Tracking of normoxic and hypoxic pretreated human Mesenchymal Stem cells in association with stroke utilizing 1H and 23Na at ultra-high magnet field MRI. Stroke, In preparation Supported by AHA Grant-in-Aid to TengMa & Samuel Grant

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