Tph1

1. 70kDa Tph1 CYP1B1 55kDa α-tubulin 50kDa α-tubulin 50kDa Cont 0.3µM Dfen 3µM Dfen 3µM Dfen 0.3µM Dfen Cont 3µM Dfen Cont 0.3µM Dfen 3µM Dfen Cont 0.3µM Dfen Suppl Figure 1. Western blots showing expression of CYP1B1 and Tph1 in human pulmonary artery endothelial cells (hPAECs) in response to Dfen. A: Dfen has no effects on CYP1B1 protein expression in hPAECs. B: Dfen administration shows a trend towards increased Tph1 protein expression in hPAECs but this was not statistically significant. Figure 2 for Reviewers. Western blots showing expression of CYP1B1 and Tph1 in human pulmonary artery endothelial cells (hPAECs) in response to Dfen or 17β-oestradiol (E2). A: Dfen has no effects on CYP1B1 protein expression in hPAECs. B: Dfen administration shows a trend towards increased Tph1 protein expression in hPAECs but this was not statistically significant. C&D: 17β-oestradiol has no effects on CYP1B1 or Tph1 protein expression in hPAECs.

2. 70kDa 55kDa CYP1B1 Tph1 α-tubulin 50kDa α-tubulin 50kDa Cont 0.1nM E2 1nM E2 1nM E2 Cont 0.1nM E2 Cont 1nM E2 0.1nM E2 Cont 0.1nM E2 1nM E2 A B Suppl Figure 2. Western blots showing expression of CYP1B1 and Tph1 in human pulmonary artery endothelial cells (hPAECs) in response to 17β-oestradiol (E2). 17β-oestradiol has no effects on CYP1B1 (A) or Tph1 (B) protein expression in hPAECs.