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The ZIRC Nursery The Basics of a Successful Zebrafish Nursery System

The ZIRC Nursery The Basics of a Successful Zebrafish Nursery System. European Zebrafish Meeting 2009 Rome, Italy. Carrie L. Barton Zebrafish International Resource Center University of Oregon. ZIRC Autonursery System. Modular rack system Fully automatic water delivery

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The ZIRC Nursery The Basics of a Successful Zebrafish Nursery System

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  1. The ZIRC Nursery The Basics of a SuccessfulZebrafish Nursery System European Zebrafish Meeting 2009 Rome, Italy Carrie L. Barton Zebrafish International Resource Center University of Oregon

  2. ZIRC Autonursery System • Modular rack system • Fully automatic water delivery • 500 ml polycarbonate tanks independent, adjustable water inlet Capacity Functional Capacity = 12,000 fish Total Capacity = 24,000 fish Density = 50 fish per tank

  3. ZIRC Nursery Feeding and Water Schedule • Water Flow Progression: Static-12hr-24hr • Food Progression and Size Overlap: Paramecia-Powder-Artemia

  4. The Fundamentals • Water Quality • Food Quantity • Food Size Goal: Embryo to Adult Effectively and Efficiently

  5. Water Quality • Pre-nursery • Embryo Medium: Modified E2 • Egg Quality • Schedule • Static vs. Running Water • This will largely depend on your particular system • Flow Rate • Clean Water vs. • Food Retention/Wash Out

  6. Food Quantity • Relationship to Water Quality • Running Water = More Food • Static System = Requires More Precise Food Delivery • Number of Feedings Per Day • 2-3 is Ideal • Amount Administered at Each Feeding • Density of Fish/Size of Tank • Avoid Overfeeding • Overfeeding = Excessive Food Build Up = Poor Water Quality • Powdered Feed and Artemia Can Adversely Effect Your Water Quality if Over Fed • Paramecia and Rotifers Can be Fed More Liberally

  7. Food Choice Commonly Use Foods: • Paramecia • Rotifers • Artemia Nauplii • Powdered Larval Diets

  8. Live vs. Powdered Feeds

  9. Food Size Food Particle Size vs. Fish Mouth Size

  10. Food Size Appropriate Size Range and Overlap Paramecium Caudatum < Brine Shrimp (SFB) < Powdered Feeds 250 x 50 µm < 400 x 200 < 100-450 (Diameter)

  11. Take Home Message The Three Goals • Water Quality • Food Quantity • Food Size -Be Ready and Willing to Observe and Make Modifications- “All Roads Lead to Rome.” Translation: Different Paths Can Take One to the Same Goal.

  12. Contact Information Zebrafish International Resource Center University of Oregon Web Address: www.zebrafish.org Email: zirc@zebrafish.org Phone: (541) 346-6028

  13. Environmental Parameters • Lighting: 14 hours/day • Temperature: 28.5˚C (22-30˚C tolerated) • pH: 7.2 to 7.6 • Conductivity: 500 -1500 µS • Ammonia: 0 ppm • Nitrite: 0 ppm • Nitrate: < 10 ppm • Exchange rate: 10% per day (RO water)

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