Task 1: Compilation of Pre-existing Knowledge About Egg and Juvenile Observation

1. Task 1: Compilation of Pre-existing Knowledge About Egg and Juvenile Observation INTERREG IVA Project Background photo by kind permission of Paul Naylor

2. Task 1.1: Creation of a geodatabase, compiling known spawning and juvenile locations

3. Explanation of database field names Insert screen shot of database here

5. Task 1.2: Population of database National datasets • Marine survey data (Professional) held by MarLIN (includes some CEFAS data) • Marine Life Survey Data (collected by volunteers) collated by MarLIN • Marine Nature Conservation Review (MNCR) and associated benthic marine data held and managed by JNCC • Seasearch Marine Surveys, provided by Marine Conservation Society • Volunteer sightings data held by the DASSH Data Archive Centre • Mollusc (marine) data for Great Britain and Ireland, provided by Conchological Society of Great Britain & Ireland Literature • Specific references to eggs and juveniles from the literature are being researched and entered • Both published and unpublished reports

6. National records are a mixture of adult, juvenile and eggs records • Adult records useful for identifying cuttlefish “hotspots” • Useful for identifying potential sampling locations

7. Lyme Bay and West Sussex have biotope maps

8. Task 1.2: Population of database New Records: Hotline: 01752 255026 Text: 07806 938789 Email: CRESH@mba.ac.uk • Where and when • Whether male, female, juvenile or eggs. • Behaviour • Substrata/habitat

9. Task 1: Next Steps • Complete literature survey (IB, All to send relevant papers and grey literature) • Combine UK with electronic records from the French coast (All to take a copy of the database template) • Include data from other CRESH tasks including our own standardised surveys • Confidence assessment • Temporal and spatial analysis

10. Task 1.3: Preparation of task summary report and dissemination of results Delivery date: 31/12/2009 • Is it possible to revise the delivery date for this task in line with Task 3?

11. Task 2: In-Situ observations of natural substratum where spawning occurs INTERREG IVA Project Background photo by kind permission of Paul Naylor

12. Poster prepared by Catherine Wilding at MarLIN to engage stakeholders and encourage the reporting of sightings. • All CRESH partners please check you are happy with the display of your logo

13. …and already we have photos sent in Many thanks to Dominic Flint, Paul Naylor, Steve Trewellha, Paul Parsons , Sally Sharrock, Matt McHugh and many more

14. Task 3: Spatial Analysis of Potential Spawning Areas INTERREG IVA Project Background photo by kind permission of Paul Naylor

15. Task 3.1: Literature review • Currently being undertaken, focusing on the factors known or thought to influence the spawning of Sepia officinalis and the survival of juveniles.

16. Task 3.2: Collate data on Cephalopod egg locations and environmental variables and habitat structure Part 1: Distributional data: From both the CRESH geodatabase and the CRESH experimental surveys (UK surveys to commence in 2010). Part 2: Environmental data: Datasets on sea surface temperature (SST), substrate particle matter (SPM), depth, substrate, salinity, exposure and currents will be collated. Whilst the location of data sources is already known for the Lyme Bay area, this will need to be investigated for the remaining sites on both the French and English coasts.

17. Task 3.3: Spatial analysis and predictive modelling of spawning habitat • Predictive modelling will be undertaken using the environmental and distribution data from Tasks 1 & 3 to predict potential spawning areas within the English Channel • Low resolution to ascertain broad trends over the area of the English Channel as a whole and finer scale resolution to investigate links within our specific sites • Possible approaches include: • Generalised additive models- which require presence/absence data 2) Maximum Entrophy model(Maxtent)- which requires only presence data

18. PhD:Topic and preliminary plans INTERREG IVA Project Background photo by kind permission of Paul Naylor

19. Topic: An evaluation of the relative importance of seagrass habitats in the recruitment of the cuttlefish Sepia officinalis

20. Objectives • Compilation of pre-existingknowledge about egg and juvenile observation. • Spatial analysis of potential spawning areas • Assessing the factors responsible for successful recruitment in terms of spawning/nursery habitat (provision of food and refuge) 4) Identify measures for the protection of cuttlefish spawning and nursery habitats

21. Methods: • Literature review – current knowledge on factors affecting spawning and juvenile survival. • In-Situ observations of natural substratum where spawning occurs – e.g. diving surveys • Laboratory experiments- e.g. egg viability (in terms of practical application to fisheries) and habitat choice. Eggs and juveniles will be collected from the study sites (and samples provided for Task 4). Collection of juveniles will also be attempted (using light traps) for a similar purpose. • Tracking juvenile origin with Stable isotope analysis in collaboration with university of Caen- by providing samples from our sites on the English coast. There is also the opportunity to apply for separate NERC funding to provide extra capacity to look at this specifically from a seagrass perspective. • Tagging studies to track spawning individuals- e.g. Vemco VRAP

22. Tagging • Option 2: • Tracking fine scale movements of juveniles within the inshore juvenile habitats • Option 1: Tracking large scale movement of individuals from Offshore to inshore From www.vemco.com