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Supplemental Figure 1. Melt Calibration using one signal and a hair-pin oligonucleotide. A) Raw melt profiling data where the calibration signal and the SMA test signal are identified. B) Analyzed SMA test signal without calibration. Note the poor clustering of profiles with the same genotypes. C) Analyzed SMA test signal with calibration. Note the tighter clustering of profiles with the same genotypes Calibration Signal SMA Test Signal