1 / 4

OPTICAL CRYOIMAGING OF KIDNEY AND THE EFFECT OF SALT INTAKE IN SALT-SENSITIVE RATS

OPTICAL CRYOIMAGING OF KIDNEY AND THE EFFECT OF SALT INTAKE IN SALT-SENSITIVE RATS. Fahimeh Salehpour , Mohammad MasoudiMotlagh,Meredith M Skelton,Allen R. Cowley ǂ , Mahsa Ranji * *Department of Electrical Engineering, University of Wisconsin - Milwaukee

dash
Download Presentation

OPTICAL CRYOIMAGING OF KIDNEY AND THE EFFECT OF SALT INTAKE IN SALT-SENSITIVE RATS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. OPTICAL CRYOIMAGING OF KIDNEY AND THE EFFECT OF SALT INTAKE IN SALT-SENSITIVE RATS FahimehSalehpour, Mohammad MasoudiMotlagh,Meredith M Skelton,Allen R. Cowleyǂ, MahsaRanji* *Department of Electrical Engineering, University of Wisconsin - Milwaukee ǂDepartment of physiology, Medical College of Wisconsin, Milwaukee, WI 53226

  2. OBJECTIVEAND INTRODUCTION • Hypertension is the biggest risk factor for the development and progression of stroke, heart failure, and end-stage renal disease. • Excess dietary salt consumption plays a causal role in the development of salt-sensitive hypertension and renal oxidative stress and injury. • In this study we used fluorescence imaging in cryogenic temperature to evaluate whether the increased consumption of salt contributes to excess oxidative stress and salt-sensitive hypertension.

  3. 10 6 8 7 4 9 3 1 METHODOLOGY 5 A/D Digital I/O Board Frame Grabber • Lens • Stepper Motor • CCD Camera • Tissue Block • Microtome Blade • Excitation Filter Wheel • Emission Filter Wheel • Ultra Low Freezer • Excitation Source • Power Supply 2 • NADH and FADautofluorescent images from each group of kidneys, were processed to extract NADH RR values using MATLAB. • Composite images were created using all image slices from cryoimager for each tissue, for both NADH and FAD signals. • The image stacks were analyzed in MATLAB to measure the 3-D max projection NADH RR of tissue and their histograms.

  4. RESULTS AND CONCLUSION Histograms Salt-sensitive Salt-resistance Mean = 106.55 Std = 23.973 NADH NADH Mean = 135.933 Std = 50.45 28% FAD Mean = 1.993 Std= 0.639 NADH Redox NADH RR Mean = 3.252 Std= 1.326 63%

More Related