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  1. 10 kb 10 kb 10 kb 20 kb 20 kb 20 kb 30 kb 30 kb 30 kb 40 kb 40 kb 40 kb 50 kb 50 kb 50 kb 60 kb 60 kb 60 kb 70 kb 70 kb 70 kb 80 kb 80 kb 80 kb 90 kb 90 kb 90 kb FIDEL I FIDEL I FIDEL II FIDEL II 1 1 2 2 3 3 4 4 5 5 Supplement S4:Schematic annotation of the BAC ADUR185P1 sequence (original sequence shown in supplement S2; GenBank accession no. GU480450). 1: position 25-629: similar to Athila ORF1 (4e-28); LCDLGA-motif (protease of Athlia) present; no significant nucleotide similarities to FIDEL element; high similarity to region 33764-33227 (position 3) (e-127). 2: position 23441-20274, Frame –3: similar to retrotransposon Gag protein (Asparagus); all conserved motifs of Athila/Calypso reverse transcriptase present; no significant nucleotide similarities to FIDEL. 3: position 33729-33290, Frame –1: similar to Athila ORF1 (1e-30); LCDLGA-motif (protease Athila) present; no significant nucleotide similarities to FIDEL; high similarity to region 25-629 (position 1) (e-127). 4: position 46586-44971, Frame –2: domain for retrotransposon Gag protein present; no significant nucleotide similarities to FIDEL; similarity of region 44965-45055 to region 1-90 (reverse) (6e-25). 5: position 70119-69642, Frame –1: similar to retrotransposon Gag protein (Asparagus) (5e-27); LCDLGS-motif of Athila5-1 present. FIDEL I: position 33765-44964 (11199 bps); FIDEL II: position 70129-81451 (11322 bps)

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