DNA: The Molecule of Heredity''

1. Chapter 11 At a Glance 11.2 What Is the Structure of DNA? 11.3 How Does DNA Encode Genetic Information? 11.4 How Does DNA Replication Ensure Genetic Constancy During Cell Division? 11.5 What Are Mutations, and How Do They Occur?

2. DNA: The Molecule of Heredity Ch. 11

3. 11.2 What Is the Structure of DNA? Knowing that DNA is made up of genes does not provide an answer to the critical questions about inheritance The secrets of DNA function and, therefore, of heredity itself are found in the three-dimensional structure of the DNA molecule

4. Cell Division Transmits Hereditary Information to Each Daughter Cell Chromosome: consists of DNA and proteins which organize its 3-D structure and regulate its use Genes: unit of inheritance; segments of DNA that range in length of #’s of nucleotides • spell out instructions for making proteins of a cell

5. 11.2 What Is the Structure of DNA? DNA is composed of four nucleotides DNA is made of chains of small subunits called nucleotides Each nucleotide has three components A phosphate group A deoxyribose sugar One of four nitrogen-containing bases Thymine (T) Cytosine (C) Adenine (A) Guanine (G)

6. Figure 11-3 DNA nucleotides phosphate phosphate base  thymine base  adenine sugar sugar phosphate phosphate base  cytosine sugar sugar base  guanine

7. 11.2 What Is the Structure of DNA? DNA is composed of four nucleotides (continued) In the 1940s Erwin Chargaff, a biochemist at Columbia University, analyzed the amounts of the four bases in DNA from diverse organisms He discovered a consistency in the equal amounts of adenine and thymine, and equal amounts of guanine and cytosine for a given species, although there was a difference in proportion of the bases This finding was called “Chargaff’s rule”

8. 11.2 What Is the Structure of DNA? DNA is a double helix of two nucleotide strands In the 1940s, several other scientists investigated the structure of DNA Rosalind Franklin and Maurice Wilkins studied the structure of DNA crystals using X-ray diffraction They bombarded crystals of purified DNA with X-rays and recorded how the X-rays bounced off the DNA molecules The resulting pattern does not provide a direct picture of the DNA structure, but the researchers were able to extract specific information

9. 11.2 What Is the Structure of DNA? DNA is a double helix of two nucleotide strands Wilkins and Franklin deduced the following information about DNA from the patterns they found: 1. A molecule of DNA is long and thin, with a uniform diameter of 2 nanometers 2. DNA is a helical, twisted like a Corkscrew or a spiral staircase 3. DNA is a double helix 4.DNA has repeating subunits 5. Phosphates are probably on the outside of the helix

10. 11.2 What Is the Structure of DNA? DNA is a double helix of two nucleotide strands (continued) James Watson and Francis Crick combined the X-ray data with bonding theory to deduce the structure of DNA They proposed that a single strand of DNA is a polymer consisting of many nucleotide subunits Within each DNA strand, the phosphate group of one nucleotide bonds to the sugar of the next nucleotide in the same strand The deoxyribose and phosphate portions make up the sugar-phosphate backbone

11. 11.2 What Is the Structure of DNA? DNA is a double helix of two nucleotide strands (continued) The nucleotide bases protrude from the sugar-phosphate backbone All the nucleotides within a single DNA strand are oriented in the same direction, and thus have an unbonded sugar at one end and an unbonded phosphate at the other end

12. 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix Watson and Crick’s findings provided the following insight about the DNA model: The DNA model consists of two DNA strands, assembled like a twisted ladder The bases protrude inward toward each other from the sugar-phosphate backbone like rungs on a ladder Hydrogen bonds hold the base pairs together, composing the rung

13. 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix (continued) The two strands in a DNA double helix are said to be antiparallel; that is, they are oriented in opposite directions From one end of the DNA molecule, if one strand starts with the free sugar and ends with the free phosphate, the other strand starts with the free phosphate and ends with the free sugar

14. Francis Crick & James Watson: • combined X-ray data with other research • and built the first double helix model • of DNA (3/7/53) • single strand of DNA is a polymer • of many nucleotide subunits • sugar-phosphate backbone • strands are antiparallel • (see next slide) • Watson, Crick, & Wilkins received • Nobel Prize in ’62 • Franklin died in ’58 so she was not • included in award

15. 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix (continued) Because of their structures and the way they face each other, adenine (A) bonds only with thymine (T) and guanine (G) bonds only with cytosine (C) Bases that bond with each other are called complementary base pairs Thus, if one strand has the base sequence CGTTTAGCCC, the other strand must have the sequence GCAAATCGGG

16. Antiparallel strands • 1 end  ‘free’ or unbonded • phosphate (5’) • 1 end  ‘free’ or unbonded • sugar ) (3’) • Complementary base pair • & Chargaff’s Rule • #A = #T • #C = #G • http://www.dnalc.org/view/15495-Chargaff • Size of bases • A & G – 2 fused rings • (large-Purines) • C & T - single rings • (small – Pyrimidines) • rungs are same width – constant diameter

17. Hydrogen bonds between complementary bases hold 2 DNA strands together

18. 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix (continued) Complementary base pairing explains Chargaff’s rule that for a given molecule of DNA, adenine equals thymine and guanine equals cytosine Since every adenine, for example, is paired with a thymine, no matter how many adenines are in the DNA molecule, there will be an equal number of thymines

19. 11.2 What Is the Structure of DNA? Hydrogen bonds between complementary bases hold two DNA strands together in a double helix (continued) Adenine and guanine are large molecules; thymine and cytosine are relatively smaller Because base pairing always places a large molecule with a small one, the diameter of the double helix remains constant In 1953, James Watson and Francis Crick consolidated all the historical data about DNA into an accurate model of its structure

20. Figure 11-5 The Watson-Crick model of DNA structure nucleotide nucleotide free phosphate free sugar phosphate base (cytosine) sugar hydrogen bonds free sugar free phosphate Hydrogen bonds hold complementary base pairs together in DNA Two DNA strands form a double helix Four turns of a DNA double helix

21. BUILD DNA http://learn.genetics.utah.edu/content/molecules/builddna/

22. Quiz nucleic acid dna structure

23. How Does DNA Replication Ensure Genetic Constancy During Cell Division? • Rudolf Virchow (1850’s): • “All cells come from pre-existing cells” • Cells reproduce by dividing in half • Each of the 2 daughter cells gets an exact • copy of the parent cells genetic • information • DNA replication = duplication of the • parent cell DNA

24. Animation: DNA Replication

25. Figure 11-6 Basic features of DNA replication Parental DNA double helix The parental DNA is unwound New DNA strands are synthesized with bases complementary to the parental strands free nucleotides Each new double helix is composed of one parental strand (blue) and one new strand (red)

26. How Does DNA Replication Ensure Genetic Constancy During Cell Division? DNA replication produces two DNA double helices, each with one original strand and one new strand (continued) The two resulting DNA molecules have one old parental strand and one new strand (semiconservative replication) If no mistakes have been made, the base sequences of both new DNA double helices are identical to the base sequence of the parental DNA double helix

27. Figure 11-7 Semiconservative replication of DNA One DNA double helix DNA replication Two identical DNA double helices, each with one parental strand (blue) and one new strand (red)

28. DNA replication produces 2 DNA double helices each with • 1 original strand and 1 new strand • Complementary base pairing provides a model for how DNA replicates • Ingredients for replication: • Parental DNA strands • Free nucleotides • Variety of enzymes to unwind • parental DNA and synthesize new • DNA strands

29. DNA helicase: enzyme that pulls apart parental DNA double helix at H-bonds btwn complementary pairs • DNA polymerase: enzyme that pairs free nucleotides with their complementary nucleotide on each separated strand Replication fork https://www.youtube.com/watch?v=5qSrmeiWsuc

30. Since DNA polymerase always moves from 3’ (sugar-end) to 5’ (phosphate-end) and DNA strands are antiparallel, DNA polymerase molecules move in opposite directions. • Short lagging strands are synthesized while the helicase continues to unwind in the opposite direction • DNA ligase: • enzyme that ties • DNA together at 9min mark – lagging strand replication

31. How long does DNA replication take? • Human chromosomes range from 50mill nucleotides in the Y chromosome to 250mill nucleotides in Chromosome 1. • Eukaryotic DNA copied at 50 nucleotides/sec; takes 12-58 days to copy a human chromosome in one continuous piece. MAKE SENSE? EFFICIENT? • Several DNA • helicases & DNA • polymerases • work to split • and copy small • pieces of the • DNA strand at • the same time.

32. Semiconservative replication: 2 resulting DNA molecules have 1 old parental strand and 1 new strand • If no mistakes have been made, the base sequence of both new strands are IDENTICAL to the base sequence of the parental DNA

33. DNA paper lab

34. 11.5 What Are Mutations and How Do They Occur? • mutations: infrequent changes in the nucleotide sequence that result in defective genes • often harmful- can cause organism to die quickly • Some have no functional effect • Some may be beneficial and provide an advantage to an organism in certain environments (basis for evolution?)

35. Accurate replication and proofreading produce almost error-free DNA • DNA polymerase mismatches nucleotides once every 1,000 to 100,000 base pairs • Completed DNA strands contain only about 1 mistake in every 100 mill to 1 bill base pairs • In humans, this amounts • to less than 1 error / • chromosome / replication • Toxic chemicals & • radiation can also • alter/damage DNA

36. Types of mutations • Point mutations (nucleotide substitutions): changes to individual nucleotides in the DNA sequence • Insertion mutations: when 1 or more new nucleotide pairs are inserted into the • DNA double helix • Deletion mutations: • when 1 or more • nucleotide pairs are • removed from the • double helix

37. Types of mutations • Inversion: when a piece of DNA is cut out of a chromosome, turned around, and re-inserted into the gap • Translocation: when a chunk of DNA (usually large) is removed from 1 chromosome and attached to another

38. Figure 11-8a Nucleotide substitution Nucleotide substitution original DNA sequence substitution nucleotide pair changed from A–T to T–A

39. Figure 11-8b Insertion mutation Insertion mutation original DNA sequence T–A nucleotide pair inserted

40. Figure 11-8c Deletion mutation Deletion mutation original DNA sequence C–G nucleotide pair deleted

41. Figure 11-9a Inversion Inversion original DNA sequence breaks DNA segment inverted

42. Figure 11-9b Translocation Translocation original DNA sequences break DNA segments switched break