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China Status Report

China Status Report. De-Zhu Li 5 July 2013. iBOL International Scientific Collaboration Committee Meeting. iFlora conceptual product. China and Southeast Asia are among the bio- diversity hotspots There are 31 000 species of vascular plants in China.

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China Status Report

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  1. China Status Report De-Zhu Li 5 July 2013 iBOL International Scientific Collaboration Committee Meeting iFloraconceptual product

  2. China and Southeast Asia are among the bio- diversity hotspots • There are 31 000 species of vascular plants in China Myers et al. 2000. Nature, 403: 853-858

  3. China joined iBOL and became a central node in April 2008

  4. 2008年10月14日 China-Canada meeting, Oct 2008 • China National Committee for Barcode of Life established in June 2008 • China-Canada joint meeting on Barcode of Life in Beijing, October 2008 • CBOL Beijing meeting, May 2009 CBOL Beijing meeting, May 2009

  5. China plant DNA barcoding pilot meeting, 3 May 2009, Beijing China plant DNA barcoding meeting, 11 July 2009, Beijing

  6. Germplasm Bank of Wild Species (GWOBS) passed national acceptance in Nov 2009 • 57618 accessions of seeds of >8141 species, and 7000 DNA samples by Dec 2012; • Good base for plant DNA barcoding • Voucher specimens for some seed collections with only vegetative characters; • Big challenges for species identification • The rapid, efficient and accurate species identification with DNA barcodes for these collections is one of the key tasks for GBOWS.

  7. Project: Plant DNA barcoding of China based on Germplasm Bank of Wild Species • Research period: July 2009-Dec. 2012 • Project number: 2009-LSF-GBOWS-01 • Participant: More than 50 botanists from 22 organizations Kick-off meeting for the project14th Aug., 2009

  8. Main research contents • To construct standardized methods and protocols for DNA barcoding • To evaluate DNA barcodes for Plant DNA barcoding • To generated DNA barcodes for the seeds in GBOWS • To generate the standardized DNA barcodes for key and represented taxa • To construct DNA barcodes database system.

  9. Project task and objectives • First stage (2009-2012) • to generate 72 000 barcodes for 4 500 species, which include 3 600 DNA barcodes for 2 000- 3000 species from GBOWS . • Second stage (2013-2016) • to obtain 14 400- 150 000 DNA barcodes for 9 000 -10 000 spp, which will facility the rapid and accurate species identification for more than one third of Chinese vascular plants.

  10. Project progress • Prepare the methods and protocols for plant DNA barcoding • Two training workshops in January and August 2010 in Kunming Plant DNA barcoding technique training

  11. Jan. 5, 2010 Planning meetings • Discussed the developing strategy of plant DNA barcoding in China after the Mexico meeting. • Attained more DNA barcodes data in a short period; • Evaluation the four DNA barcodes; • To publish ‘high- impact’ papers.

  12. Jan. 2010, Field expedition 24 people, 9 organizations Southeastern Yunnan Seed plant: 1379 records; 3 specimens and 2 DNA samples for each individual Bryophyte: 426 records, 60 % to species

  13. Two papers favored ITS2 published by a group lead by Chen Shi-Lin of the Institute of Medicinal Plant Development (IMPLAD), Beijing in 2010

  14. DNA barcodes evaluation based on a large dataset • A total of 6286 individuals of 1757 species (1675 angiosperms and 82 gymnosperms) were used for universality analyses, and a total of 21673 sequences were obtained (5826 for rbcL,5471 for matK, 5566 for trnH-psbA, and 4810 for ITS); • ITS was suggested to be part of the core barcode for plants

  15. Community phylogeney of Dianhushan 20 ha forest dynamic plot with DNA barcoding 183 species rbcL, trnH-psbA, and matK： 1–2 individuals Phylogenetic clustering : valley and low-slope habitats Phylogenetic over-dispersed: high-slope, ridge-top and high-gully habitats More accurate community phylogenetic tree with DNA barcodes, To reveal the real coexistent and assemblage mechanism of community. Pei et al. 2011. PLoS One 6: e21273

  16. 13 articles of plant DNA barcoding published in this special issue; • All of them were supported by the grant from the Large-Scale Scientific Facilities of the Chinese Academy of Sciences (No. 2009-LSF-GBOWS-01).

  17. Other projects • Animal DNA Barcodes and Cryptic Diversity, an NSFC key-note project (10 million CN Yuan, Jan.2011 – Dec. 2014), PIs: Ya-Ping Zhang, Da-Wei Huang, Jing Che and Shun-Ping He • Technical Standards and Information System for DNA barcoding in China (10 million CN Yuan, July. 2011 – June 2015), PI: Ge-Xia Qiao • DNA barcoding in Some Natural Reserves (10 million CN Yuan, July 2012 – June 2016), PI: Douglas Yu • Digitalization of the Flora Reipublicae Popularis Sinicae and DNA barcoding (10 million CN Yuan, July 2013 – June 2017), Pis: Xian-Chun Zhang & Hong Wang

  18. Barriers and threats • DNA barcoding is becoming a fashion in many areas in China but some do not understand the principles of DNA barcoding • China is a centralized country but with dicentralized funding and different funding agencies have different criteria • Lack of qualified taxonomists and many young students play DNA barcoding without a proper training in taxonomy • Problems in getting data from other parties

  19. China signs iBOL MOU to consolidate its central node status 31 May 2012

  20. The 5th International Barcode of Life Conference Kunming, China 27- 31 Oct. 2013 http://www.dnabarcodes2013.org Call for papers, symposium and workshop proposal

  21. Thank You！

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