1 / 22

BIO 201 GENETICS Lecture #37 “Structural Genomics” Dr. Karen Schmeichel November 21, 2008

BIO 201 GENETICS Lecture #37 “Structural Genomics” Dr. Karen Schmeichel November 21, 2008. DNA Forensics lab report due 11/24 Lab 11/24: Ames Test Bring in an experimental material to test 12/1 Visiting speaker: Jacob Kagey. Business Items:. Objectives:.

debbie
Download Presentation

BIO 201 GENETICS Lecture #37 “Structural Genomics” Dr. Karen Schmeichel November 21, 2008

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. BIO 201 GENETICSLecture #37“Structural Genomics” Dr. Karen SchmeichelNovember 21, 2008

  2. DNA Forensics lab report • due 11/24 • Lab 11/24: Ames Test • Bring in an experimental • material to test • 12/1 Visiting speaker: • Jacob Kagey Business Items:

  3. Objectives: • Finish our discussion of Gene therapy • Discuss Genomics as the study of genes in • the context of entire genomes • Understand the Mapping approach to • Genomic sequencing as building a • complete outline before writing a paper • Compare this to Whole Genome shotgun • sequencing which relies more on • computer-assisted analysis

  4. WHAT ABOUT GENE THERAPY?

  5. CASE STUDY: Sometimes It Is All In the Genes “Part IV”

  6. Considering Entire Genomes Other Applications

  7. GENOMICS: The development and application of new mapping, sequencing and computational procedures to analyze the entire genome of organisms

  8. Other Applications • Genomics: • 3 Categories • Structural • Functional • Comparative

  9. Fig. 23.1/4 First Genome Sequenced: Human Mitochondria

  10. Human Genome Project Goals: • Identify all human genes and • comparative seqs. in model orgs. • Sequence 3.1 billion bases • Data >> public access • Transfer info to private/public • Address ethical issues SEE: www.ornl.gov/TechResources/Human_Genome/home.html

  11. Private Genomic Sequencing J. Craig Venter Celera Genomics http://www.pbs.org/kcet/wiredscience/video/289-craig_venter.html

  12. The Sequencing Race Proposed: 1986 Finished: 2003

  13. 2 Approaches to Sequencing Genomes: • Mapping • Whole-Genome • Shotgun

  14. A. Fig. 16.12 Step 1: Generate a High-density Genetic Map

  15. Map by: • Gene • By Unique Restriction Enzyme • STS – Unique sequence tag site • ESTs – expressed RNAs • Fish to map the fragment on • the chromosome .

  16. A. Fig. 10.1 Step 2: Physical Mapping of YAC/BAC Contigs

  17. A. Step 3:Sequence BAC clones Limited to ~ 500 bp/reaction

  18. 2 Approaches to Sequencing Genomes: • Mapping • Whole-Genome • Shotgun

  19. Private Genomic Sequencing DNA Prep DNA Sequencers

  20. B. Fig. 10.2 Whole Genome Shotgun (WGS) Approach ANIMATION Geneticsplace.com Ch. 10 (18) “Direct Shotgun Sequencing of Genomes”

  21. Take-Home Points: • Genomics is the study of the structural and functional elements of an entire genome • HGP and Celera genomics used two different approaches to derive genomic DNA sequences from human sources • Genomic information is now being used to understand the essence of life – asking questions about can we reproduce it in the lab?

  22. Assignments: New Quiz Material: Functional and Comparative Genomics 10:251-265 (or 18: 495-509) Problems: Ch 10: 1, 2, 6, 7, 13, 14 (same in Ch 18 of “Mendelian”)

More Related