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Comparative Analysis of Arbutin and T ranexamic Acid in Skin W hitening P roducts

Comparative Analysis of Arbutin and T ranexamic Acid in Skin W hitening P roducts. Chuxin Chen 12-07-2011. Backgrounds.

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Comparative Analysis of Arbutin and T ranexamic Acid in Skin W hitening P roducts

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  1. Comparative Analysis of Arbutin and Tranexamic Acid in Skin Whitening Products Chuxin Chen 12-07-2011

  2. Backgrounds • According to the aesthetic of most estern Asian people, women are more attractive in brighter skin tones. Thus the skin whitening products have a large market in countries like Japan, Korea and China. • Different types of whitening products varied a lot in prices, I would especially investigate in how the tranexamic acid added products are different form the arbutin added products.

  3. Analytes

  4. Hypothesis • The hypothesis is: the tranexamic acid added products are more effective, stable and healthier than the arbutin added products.

  5. Reverse-Phase HPLC MS • The reverse-phase HPLC will give good peak resolutions and high efficiency coupled to tandem MS • Able to quantify analytes by using a internal standard solution.

  6. Instrumentation • HPLC: Agilent 1200 • Column: Nucleosil brand C18 (10µm,25*0.46cm) • The mobile phase for the separation was acetonitrile/water (v:v 50:50), the PH of the mobile phase was adjusted to 2.6 by phosphoric acid (85%, w/w). • Detector: tandem MS

  7. Sample preparation • 10ml of the internal standard solution (0.5g/L) is made and added into the 100µL sample which was contained into a 1.5mL plastic tube. • 100µL of perchloric acid(2.5% w/w) were than added. • sample was then vortex mixed for 30s and centrifuged at 14,000rpm for 10min. • 100µL of the aqueous supernatant is decanted into another tube and 150µL of sodium hydroxide (0.1M) was added. • sample is vortex mixed for 10s, and transferred into injection vials for analysis.

  8. Results MS of tranexamic acid derivative of Ethyl chloroformate MS of arbutin transfer product by rBglA

  9. Method specifications: • Concentration range: 1.0-200.0 µg/mL • linear regression correlation coefficient: 0.9991 • CV range: 0.7-8.6% • LOD: 0.05 µg/mL • LLOQ: 1.0 µg/mL • ULOQ : 200.0 µg/mL • Accuracy: 96.9%

  10. Alternative methods

  11. Methods for other comparisons

  12. References: • No time left….. (＞﹏＜)

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