40S

1. Supplementary Information – Sangthong et al., 2007 B A PTC 209 PTC 208 50 ng/ml doxy 1 g/ml doxy 80S 1 g/ml doxy polysomes 60S 40S (P/M ratio = 1.69) (0.42) (0.22) (0.08) (1.75) D PTC 229 C PTC 210 50 ng/ml doxy 1 g/ml doxy 50 ng/ml doxy 1 g/ml doxy (0.02) (0.14) (0.08) (0.74) (0.12) (0.09) E PTC 230 50 ng/ml doxy 1 g/ml doxy (0.36) (0.20) (0.18) Polysome gradient analysis of strains PTC 208, 209, 210, 229 and 230. Sucrose gradients were loaded with cell extracts derived from the respective strains grown in SD-met medium [containing doxycycline (50 ng/ml or 1 g/ml) where indicated] for 16 hours. Subsequent to centrifugation, and fractionated while scanning at A254 (as described in reference 1). The positions of the 40S and 60S subunits, 80S ribosomes, and polysomes are indicated. Polysome to monosome ratios (P/M ratio) were calculated by integrating the area under the polysome peaks and the 80S peak using Image J version 1.37. 1. Vasilescu, S., Ptyushkina, M., Linz, B., Müller, P.P. and McCarthy, J.E.G. (1996) Mutants of eukaryotic initiation factor eIF4E with altered mRNA cap binding specificity reprogram mRNA selection by ribosomes in Saccharomyces cerevisiae. J. Biol. Chem.271, 7030-7037.