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Dynatech MR5000

The Dynatech MR5000 is a microprocessor-controlled photometer that supports various tasks using 96-well microplates. With features like single and dual wavelengths and five different filters, it eliminates errors and enables advanced applications. From kinetics to blood grouping, the MR5000 is perfect for a range of assays. This device is particularly useful for tasks such as the Bradford assay, ELISA, and homogenization. It provides accurate protein concentration measurements, ensuring consistent results for experiments. Order the Dynatech MR5000 today and experience its wide range of capabilities.

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Dynatech MR5000

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  1. Dynatech MR5000 Using a Microplate Reader for a variety of tasks

  2. Features of the Dynatech MR5000 • Microprocessor controlled photometer • 96-well microplates • MICROELISA • MICROTITER • 5 different filters • 405, 450, 490, 550, 595 (nm) • Single and dual wavelengths • eliminates some error

  3. Applications • Kinetics • Agglutination • Tissue culture growth • Blood grouping • Complement fixation • MIC and bacterial ID assays • Advanced applications

  4. What I do with the MR5000 • Bradford Assay • total protein concentration of a sample • quick and easy • ELISA - more involved than Bradford • Enzyme-Linked Immunosorbent Assay • Buffers • 1 and 2 antibodies • Color Detection

  5. Bradford Assay • Homogenization & Centrifugation • Preparation of Samples & Standards • Plate Loading and Bradford Reagant • Coomassie Brilliant Blue G-250 & H3PO4

  6. What the Bradford tells us • Total protein concentration for each sample Why is this important? • To run ELISA need same amount of protein in each sample – for my work 2000 ng • For ELISA use a particular volume for each sample (determined by Bradford readings) FEB BRADFORDS completed.xls

  7. Monoclonal Antibody ELISA • Bradford data • Standards • Reagants • Binding Buffer (10mM Na2CO3/NaHCO3) • Blocking Solution – PBST (PBS + Tween 20) • PBST-BSA • Antibodies – • Primary • Secondary • Color Detection Solution • Diethanolamine R-nitrophenylphosphate tablets DAS-ELISA

  8. HSP 70

  9. Summary • So, we have: • Determined the concentration of protein in a sample (Bradford 595 nm) • Quantified the concentration of HSP 70 in equal volumes of protein (ELISA 405 nm) • Had a lot of fun!!

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