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3 Hz. 2 Hz. A. WT. R4496C. 100 μ m. ISO. ISO. 250 ms. 150. 100. Iso effect on [Ca 2+ ] i transient (% F/F 0 of control). 50. 0. WT. R4496C. WT. WT. R4496C. R4496C. 4 Hz. B. 120. 80. Iso effect on SR Ca 2+ load (% F/F 0 of control). 40. 0. WT. R4496C.
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3 Hz 2 Hz A WT R4496C 100 μm ISO ISO 250 ms 150 100 Iso effect on [Ca 2+]i transient (% F/F0 of control) 50 0 WT R4496C WT WT R4496C R4496C 4 Hz B 120 80 Iso effect on SR Ca 2+ load (% F/F0 of control) 40 0 WT R4496C WT WT R4496C R4496C 4 Hz 3 Hz 2 Hz