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DNA microarray

DNA microarray. Research Integration. Statistics (data analysis). Biology. Microarray. Optics (signal detection). Electronics, physics. Chemistry (surface modification). Engineering (microfluidic, microfabrication, robotic automation…). Basic Research. Crop Improvement.

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DNA microarray

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  1. DNA microarray

  2. Research Integration Statistics (data analysis) Biology Microarray Optics (signal detection) Electronics, physics Chemistry (surface modification) Engineering (microfluidic, microfabrication, robotic automation…)

  3. Basic Research Crop Improvement Sequencing/resequencing Gene Expression Microarray Diagnosis Gene Discovery Drug Discovery Genotyping (SNPs) Disease Barcoding, Drug Susceptibility

  4. Collections of ESTs, library clones... 96-well plate PCR amplification PCR product purification Re-racking Quality control 384-well plate DNA printing solution Preparation of DNA solution for microarray

  5. Printing of DNA microarray Modified fromhttp://darwin.bio.uci.edu/~faculty/wagner/array2.html

  6. Each spot represents different gene/clone Modified fromhttp://darwin.bio.uci.edu/~faculty/wagner/array2.html

  7. cDNA labeling with fluorescent dyes AAAAA AAAAA AAAAA mRNA isolation AAAAA AAAAA AAAAA AAAAA AAAAA AAAAA AAAAA Flowers Leaves DNA microarray probe preparation

  8. DNA microarray hybridization Flower Leaf Modified fromhttp://darwin.bio.uci.edu/~faculty/wagner/array2.html

  9. Labeled DNA hybridizes to corresponding DNA/gene Modified fromhttp://darwin.bio.uci.edu/~faculty/wagner/array2.html

  10. Cy3: ~550 nm Cy5: ~650 nm Laser excitation Image overlay No changes Flower up-regulated genes Leaf up-regulated genes

  11. Arabdidopsis 11.5K microarray Cy3 labeled cDNA from etiolated seedlings Cy5 labeled cDNA from green leaves

  12. Detection of cross hybridization Girke et al. 2000

  13. Correlation between protein and mRNA levels Gygi et al. 1999

  14. Plant functional genomics Basic mechanism of plant growth and development Application in agricultural biotechnology 增加農作物產量 創造高營養價值之農作物 創造具高適應力之農作物 利用農作物作為生物反應器

  15. DNA microarray and beyond….

  16. Insertion site identification from knockout population Mahalingam and Fedoroff, PNAS (2001) 13, 7420-7425

  17. DNA copy number detection Pollack et al. 1999

  18. DNA-protein interaction Iyer et al. Nature (2001) 409,533-38

  19. Protein-protein interaction (protein microarray) MacBeath et al. (2000) Science 289, 1760-3

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