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Metadata Date (at beginning and end of data collection) (year, month, date)

Metadata Date (at beginning and end of data collection) (year, month, date) Growing season ? (yes or no) GPS location (in decimal degrees) In situ or pot? Species ID (family, genus, species) Plant functional type Growth form C3/C4/ Cyad Angiosperm/ gymnosperm Deciduous/evergreen

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Metadata Date (at beginning and end of data collection) (year, month, date)

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  1. Metadata • Date (at beginning and end of data collection) (year, month, date) • Growing season? (yes or no) • GPS location (in decimal degrees) • In situ or pot? • Species ID (family, genus, species) • Plant functional type • Growth form • C3/C4/Cyad • Angiosperm/ gymnosperm • Deciduous/evergreen • Contributor • Citation • Site ID • Root order • Functional classification • Soil depth ( upper and lower) (cm) • Depth of field (mm) • Treatment, extent of treatment, and unit for extent • Collection method • Soil block dimensions (length, width, height) (cm) • Calculation method • Latitude, longitude • Stand age (yr) • Location notes • notes

  2. Morphology • Root diameter (mm) • Specific root length (SRL) (m g-1) • Root tissue density (RTD) (g cm-1) • Branching intensity (# roots per higher order root) • Branching architecture (root length per higher order root) • Type of mycorrhizae (AM or EM) • % EM colonization (% tips colonized) • % AM colonization (% length colonized) • Anatomy • Stele diameter (µm) • Cortex diameter (µm) • Root porosity (aerenchyma area) • Vessel diameter (µm) • Phellem • Standing Biomass • Absolute biomass (g m-2) • % total biomass (by order, diameter, and functional class) • Annual production (g m-2) • Annual mortality (g m-2 year-1) • Biomass turnover (g m-2a-1)

  3. Chemistry • Root C, N, K, P, Al, Ca, Mg (mg g-1) • Root Cr, Ni, Cu, Zn, As, Cd, Pb(mg kg-1) • C:N ratio • C storage (kg C hm-2) • Ion concentration (Na+ and Cl-) (mg g d.wt-1) • Lignin • Cellulose • Hemi-cellulose • Carbohydrates (TNC)(mg g-1) • Isotopic concentration Mean root stable δ15N (%) • Dynamics • Survivorship (roots year-1) • Growing season survivorship (% growing season-1) • Phenology production • Phenology mortality • Lifespan (days) • Turnover rate (% growing season-1) • Function • Respiration rate (nmol O2 g-1 s-1) • Respiration rate by mass (nmol s-1 m-2) • Nutrient uptake • Water uptake • Exudate rate • Oxygenation rate • Enzyme production • Conductance

  4. Soil dynamics • Soil temperature (°C) • Volumetric soil water content (%) • Oxygen availability • Soil respiration • Mineralization rate • Climate • Mean annual precipitation (mm) • Min & max monthly temperature (°C) • Mean annual temperature (°C) • Chamber temperature (day, night) (°C) • Other site characteristics • Elevation (m above sea level) • Slope (°) • Soils • pH • Textural class • Soil horizon • % sand, silt, clay, and SOM • Bulk density (g cm-3) • Soil N and P (g kg-1) • Al:Ca ratio • Cation exchange capacity (CEC) (meq cg-1) • % base cation saturation • Elemental concentration (total and exchangeable) • Redox potential (mV) • Vegetation • Basal area (total and by species) • LAI (total and by species) • Photosynthesis

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