Biological Processes

1. Differential Gene Expression in Olympia Oyster (Ostrealurida) Gonadal Tissue Hannah Wear, Carolyn Friedman, and Steven Roberts Biological Processes School of Aquatic and Fishery Sciences, University of Washington Cellular Components Molecular Function Differential Gene Expression in Male compared to Female Background Biological Processes CellularComponents Molecular Function Gene Ontology terms associated with UniProt/Swissprot annotations Transcriptomic data provides information which can aid in conservation of species with high vulnerability, such as the Olympia oyster. The DESeq analysis figure shows the fold change of male expression levels, where positive fold change indicates a gene expressed higher in male gonad tissue, and vice versa. DESeq analysis indicates 1789 genes expressed significantly higher in female gonad tissue and 495 genes expressed significantly higher in male gonad tissue. Red dots represent differentially expressed genes with a significant p-value (<0.05). Biological Processes Cellular Components Molecular Function Research Objectives: Expand existing annotated O. lurida transcriptome resources and determine which genes have the greatest difference in expression between O. lurida gonad tissue. Approach Enrichment of Genes and Pathways 1. Transcriptome Annotation: BLAST O. lurida contigs to known gene sequences using multiple gene and protein databases and assess results with gene ontology (GO) terms. 2. Expression Analysis: Use DESeq analysis to determine differentially expressed contigs from O.lurida male and female gonad tissue. Genes with significant differential expression from DESeq analyses were subjected to enrichment analysis using DAVID analysis and categorized by GO terms into three categories: biological processes, cellular components, and molecular function. Results also showed significant differential expression in the N-glycan biosynthesis pathway. O. lurida Transcriptome Annotation GO enrichment of significantly expressed genes Conclusions These findings and tools expand the existing public genomic resources on O. lurida and provide baseline data for conservation of the Olympia oyster and related species. Project funded in part by a NOAA Aquaculture Program Award (NA10OAR4170057) and SAFS.