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MdSFBB3-alpha MSHVRESETPEDRVVEILSRLPPKSLMRFKCIHKSWFSLINNLSFVAKHLSNSVDNKLSSSTCILLNRSQAHIFPDQSWKQEVFWSMINFSIDSDENNLHYDVEDLN-IP 109 MdSFBB3-beta MSQVHESETPEDKVVEILCRLPPKSLMRFKCIRKSWCTLINRPSFVAKHLNNSVDNKLSSSTCILLNRSQAHIFPDQSWKQEVFWSTINLSIDSDEHNLHYDVEDLI-IP 109

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MdSFBB3-alpha MSHVRESETPEDRVVEILSRLPPKSLMRFKCIHKSWFSLINNLSFVAKHLSNSVDNKLSSSTCILLNRSQAHIFPDQSWKQEVFWSMINFSIDSDENNLHYDVEDLN-IP 109 MdSFBB3-beta MSQVHESETPEDKVVEILCRLPPKSLMRFKCIRKSWCTLINRPSFVAKHLNNSVDNKLSSSTCILLNRSQAHIFPDQSWKQEVFWSTINLSIDSDEHNLHYDVEDLI-IP 109 MdSFBB9-alpha MSQVRESETPEDQVVEILSRLPPKSLMRFKCIRKSWCTIINSSSFVAKHLSNSIDNKLSSSTGILLNRCQVHVFSDRSWKQDVFWSMINLSIDSDKNNLYYDVEDLN-IP 109 MdSFBB9-beta MSQVRESETLEDKVVEILSKLPPKSLMRFKCIRKSWCTIINSPSFVAKHLNNSMDYKLSSTTCILLNRCQVHVFPDRSWKQDVFWSMINLSIDSDEHNLHYDVEDIN-IP 109 PpSFBB4-alpha MSQVHESETPEDKVVEILSRLPPKSLIRFKCVRKSWCTIINSPSFVAKHLSNTVDNKFSSFTCILFNRSQVHVFADRSWKRDVFWSMINLSIESDEHNLDYDVEDLN-IP 109 PpSFBB4-beta MTQVRESETPEDRVAEILSRLPPKSLMRFKCIRKSWGTIINNPSFMAKHLSNSVDNKFSSSTSILLHRSQMPVFPDRSWKREYFWSMINLSHDSDEYNLYYDVEDLN-IQ 109 PpSFBB4-gamma MSQVRESETPEDRMVEILSRLPPKSLMRFKCIRKSWCTLINSPCFVAKHLSDSVDNKLSSSTCILLNCSQAHVCSEKSWKQEVSWSVINLSIDGDE--LHYDIEDLTIVP 108 PpSFBB5-alpha MSQVHESETPQDKVVEILSRLTPKSLMRFKCVHKSWCTIINSPSFVAKHLSNTVDDKFSSFTCILFNRSQVHVFADRSWKRDVFWSMVNLSIDSDEHNLHYDVKDLN-IP 109 PpSFBB5-beta MTQVCESETLEDRMAEILSRLPPKSLMRFKCIRKSWCTVINNPSFMAKHLSNSVDKKFSSSTCILLHRSHMPVFPDGSWKREYFWSMINLSRDSDEHNLYYDVEDLN-VQ 109 PpSFBB5-gamma MSQVRESETPEDRMVEILSRLPPKSLMRFKCIRKSWCTLINSPCFVAKHLSDSVDNKLSSSTCILLNCSQAQVCSEKSWKQEVSWSVINLSIDGDE--LHYDIEDLTIVP 108 * * **** *# *** * **** **** *** #** * **** *#* ** * ** # # #*** ** * * * # * ** * <------------------------------------------------> F-box MdSFBB3-alpha FALKDHDFVLIFGYCNGILCVEAGKNVLLCNPATREFKQLPDSCLLLPSPP-ERKFELETNFQALGFGYDCNAKEYKVVRIIEN--CEYSDDERTYYYRIALPHTAELYT 216 MdSFBB3-beta FPLEDHDFVLIFGYCNGIVCVDAGKNVLLCNPATREFRQLPDSCLLQP-PP-KGKFELETTFQALGFGYDCNAKEYKVVRIVEN--CEYSDDEQTFYHRIALPHTAEVYT 215 MdSFBB9-alpha FPMEDQDNVELHGYCNGIVCVIVGKNVLLCNPATGEFRQLPDSSLLLPLP--KGRFGLETVFKGLGFGYDCKAKEYKVVRIIENCDCEYSEGEESYYERILLPHTAEVYT 217 MdSFBB9-beta FPMEDQDNVELHGYCNGIVCVIVGKNVLLCNPATGEFRQLPNSPLLLPLP--KGRFGLETTFKGMGFGYDCKSKEYKVVRIIENCDCEYSDDGESYYERILLPHTAEVYT 217 PpSFBB4-alpha FPMEVQDNVQLYGYCNGIVCVIVGENVLLCNPATREFKQLPDSSLLLPLP--TGKFGLETLFKGLGFGYDCKTKEYKVVRIIENCDCEYSEGKESYYERILLPYTAEVYT 217 PpSFBB4-beta FPLEDHDHVSIHGYCNGIVCLIVGKNAVLYNPATRELKQLPDSCLLLPSPP-EGKFELESTFQGMGFGYDSKAKEYKVVKIIEN--CEYSDDMRTFSHRIALPHTAEVYV 216 PpSFBB4-gamma FLKDGPHEVEIHGYCDGIVCVTVDENFFLCNPATGEFRQLPDSCLLLPLPGVKEKFGLETTLKGLGFGYDCKAKEYKVVRIIDNYDCEYSEDGETYIEHIALPYTAEVYT 218 PpSFBB5-alpha FPMEVQDNVQLYGYCNGIVCVIVGENVLLCNPATREFKQLPDSSLLLPLP--TGRFGLETLFKGLGFGYDCKTKEYKVVRIIENCDCEYSEGKESYYERILLPYTAEVYT 217 PpSFBB5-beta FPLEDHEHISVHGYCNGIVCLIVGKNALLYNPATRELKQLPDSCLLLPSPP-EGKFELESTFQGMGFGYDSKAKEYKVVKIIEN--CEYSDDERTFSHRIALPHTAEVCI 216 PpSFBB5-gamma FLKDGPHEVEIHGYCDGIVCVTVDENFFLCNPATGEFRQLPDSCLLLPLPGVKEKFGLETTLKGLGFGYDCKAKEYKVVRIIDNYDCEYSEDGETYIEHIALPYTAEVYT 218 * # # # ## *** ** * # * * **** * *** * ** * * # * ** # ***** ****** * * **** # * ** *** <---------> V1 MdSFBB3-alpha TTANSWKEIKIDISSTTYS-----CSRSVFMKGFCYWYATDGEEYILSFDLGDDTFHIIQLPSRRESGFRFYYIFLRNESLASFCSRYDRSEDS---ESCEIWVMDDYDG 318 MdSFBB3-beta TAANFWKEIKIDISIKTYH-----CSCSVYLKGFCYWFASDNEEYILAFYLGDETFHIIQLPSRRESGFTFDYIFLRNESLASFCSPYNPSEDS---KLFEIWVMDDYDG 317 MdSFBB9-alpha MTADSWKEIKIDVSSDTDP-YCIPYSCSVYLKGFCYWFACDNGEYIFSFDLGDEIFHIIELPSRREFGFKFYGIFLYNESITSYCSRYE--EDC---KLFEIWVMDDYDG 321 MdSFBB9-beta MTANSWKEIKIDISIETRW-YCIPYSGSVYLNGFCYWFAYDNGEYVFSFDLGDEIFHKIDLPSRRESDFKFYGIFLYNESVTSYCYRHE--EDC---ELFEIWVMDDYDG 321 PpSFBB4-alpha TAANSWKEIKIDTSSDTDP-YCIPYSCSVYLKGFCYWFANDNGEYIFSFDLGDEIFRRIELPFRRESDFNFYGLFLYNESVASYCSRYE--EDC---KLLEIWVMDDHDG 321 PpSFBB4-beta TTTNSWRVIEIEISSDTYN-----CSCSVYLKGFCYWFASDDEKYVLSFDLGDEIFHRIQLPYRKESGFLFYDLFLYNESIASFCSHYD-NDNAGILEILEIWVMDDCDG 320 PpSFBB4-gamma MAANSWKEITIDILSKILSSYSEPYSYSVYLKGFCYWLSCDVEEYIFSFDLANEISDMIELPFRGEFGFKRDGIFLYNESLTYYCSSYE--EPS---TLFEIWVMDYDDG 323 PpSFBB5-alpha TAANSWKEIKIDTSSDTDP-YCIPYSCSVYLKGFCYWFANDNGEYIFSFDLGDEIFRRIELPFRRESDFNFYGLFLYNESVASYCSRYE--EDC---KLLEIWVMDDYDG 321 PpSFBB5-beta TTTNSWRVIEIEISSDTYN-----CSCSVYLKEFCYWFASDDEECILSFDLGHEIFHRIQLPCRKESGFLFCDIFLYNESIASFCSHYDESDNSGILKILEIWVMDDCDG 321 PpSFBB5-gamma MAANSWKEITIDILSKILSSYSEPYSYSVYLKGFCYWLSCDVEEYIFSFDLANEISDMIELPFRGEFGFKRDGIFLYNESLTYYCSSYE--EPS---TLFEIWVMDYDDG 323 * * * # ## *#** **** #*# * * #*#**#* * *# ** ***# * # # # # #****** #** <-----------------------------------------> V2 MdSFBB3-alpha DKSSWTKLLNIGPLQGIKKPLTFWRSDELLMLDSDGRATSYNYSTRNLKYLHIPPILNRVVDFEVLIYVKSIVHVK 394 MdSFBB3-beta VSSSWTKLLTVGPFKGVEYPLTLWKCDELLMLASDGRATSYNSSTGNLKYLHIPPILNKVVDFEGLIYVKSIVPLK 393 MdSFBB9-alpha VKSSWTKLLAVGPFKDIDYPLTLGKFDEVLMLGSYGRAAFCNSSTGNLKYLHIPPIIN-----WMIDYVKSIVPVK 392 MdSFBB9-beta VKSSWTKLLTIGPLKDIDYPLTLWKCDEVLMLGSYGRAASCNSSTGNLRYLHIPPIIK-----WMMDYVKSIVPVQ 392 PpSFBB4-alpha VKSSWTKLLTVGPFKDIESPSTFWKCDEVLILSSYGKATSYNSGTENLKYLHIPPIIN-----WMIDYVETIVPVK 392 PpSFBB4-beta VKSSWTKLLTLGPFEDNENLLTFWKSDELLMVTSDKRAISFNSSTGNLKYIHIPPIMNKVTDFEALIYVESFVSVK 396 PpSFBB4-gamma FKSSWTKHLTAGPFIDMEFPLTPWKRDELLMIASDGRAASYNSCTGNFKYLHIPVIINQN---RVVDYVKSIILVN 396 PpSFBB5-alpha VKRSWTKLLTVGPFKDIESPSTFWKCDEVLILSSYGKATSYNSSTGNLKYLHIPPIIN-----WMIDYVETIVPVK 392 PpSFBB5-beta VKSSWTKLLTLGPFKGNENLLTFWKSDELLIVTSDQRAISYNSSTGNRKYIHIPPIINKITDLEALIYVESIVSIK 397 PpSFBB5-gamma FKSSWTKHLTAGPFTDMEFPLTPWKRDELLMIASDGRAASYNSCTGNFKYLHIPVVINQN---RIVDYVKSIILVN 396 **** * #** # # # *# #** * #* *# * * * * *** ### ### ** # <---------------> <---------> V3 V4 Supplemental Figure 1. Amino acid sequence alignment of SFBBs for NVI analysis. Conserved sites and most variable sites with NVI values of > -0.25 were marked with asterisks and #, respectively. Double-headed arrows denote F-box and variable regions.

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