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Efficient pVCB6 expression system with novel env1 and env2 variants

Explore the enhanced expression of pVCB6Fd through the novel pVCB6Fdenv1 and pVCB6Fdenv2 constructs in different cell lines. This system offers improved efficiency and versatility for molecular studies and biotechnological applications.

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Efficient pVCB6 expression system with novel env1 and env2 variants

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  1. (A) (B) Nde I Nde I cmyc T T VH VH VL VH CH1 CL CH1 CH1 T 7 T7 T 7 T7 pVCB6LC pVCB6Fd Ori Ori F F (C) pVCB6Fdenv2C pVCB6Fdenv2S pVCB6Fdenv1C Cys Ser Ser Cys Cys Cys Ser Ser pVCB6Fdenv1S env2C TGCTGC env1C TGCTGC env1S TCCTCC env2S TCCTCC env cmyc T pVCB6Fdenv EcoR I EcoR I EcoR I Mlu I Mlu I Mlu I Mlu I Ori F Nhe I Nhe I Nhe I Nhe I Cys Cys Cys Cys pVCB6Fdenv12CC env1C TGC TGC L env2C TGC TGC Ampr Ampr Ampr Ampr pVCB6Fdenv12SC pVCB6Fdenv12CS Cys Ser Ser Ser Ser Cys Ser Cys Ser Ser Cys Ser pVCB6Fdenv12SS env1C TGC TGC L env2S TCC TCC env1S TCC TCC L env2C TGC TGC env1S TCC TCC L env2S TCC TCC EcoR I env1 L env2 cmyc T pVCB6Fdenv12 Ori F (D)

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