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Controlling Microbial Growth in the Environment

Explore effective ways to control microbial growth in the environment using physical and chemical methods. Learn about sterilization, disinfection, heat, chemicals, and more to maintain a hygienic setting. Enhance your knowledge of terminology and techniques for combating microbes.

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Controlling Microbial Growth in the Environment

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  1. Controlling Microbial Growth in the Environment

  2. Controlling Microbial Growth • Terminology of Microbial Control • Sterilization • Aseptic • Disinfection • Degerming • Sanitation • Pasteurization • Stasis/static • Cide/cidal

  3. Controlling Microbial Growth • Physical Methods of Microbial Control • Heat • Moist Heat • Boiling • Autoclaving • Pasteurization • Dry Heat • Refrigeration & Freezing

  4. Controlling Microbial Growth Contd- • Desiccation • Lyophilization • Filtration • Osmotic Pressure • Radiation

  5. Controlling Microbial Growth Contd- • Chemical Methods of Microbial Growth • Phenols and Phenolics • Denatures • Alcohols • Denatures • Halogens • Iodine used as antiseptics • Chlorine used in drinking water and as disinfecting bleach • Bromide used as a disinfectant in hot tubs because it evaporates more slowly than chlorine at high temperatures.

  6. Controlling Microbial Growth Contd- • Chemical Methods Contd- • Oxidizing Agents • Peroxides and Ozone • Reactive oxygen that kills microbes by oxidizing their enzymes and preventing their metabolism • Surfactants • Soaps and Detergents reduce the surface tension of solvents like water and disrupt cell membranes • Heavy Metals • Zn, arsenic, Hg, Cu, Ag,- alter 3-d shape of proteins

  7. Controlling Microbial Growth Contd- • Chemical Methods Contd- • Aldehydes • Compounds containing CHO group. Crosslink amino, COOH, OH Groups in proteins to denature them • Use to disinfect medical and dental equipment • Gaseous Agents • Used for sterilizing plastic labware, mattresses, pillows and things that cannot be easily sterilized by heat, chemicals or irradiation. Examples include ethylene dioxide and propylene oxide.

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