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The Cell. Honors Biology Chapter 4. Microscopes A. Magnification – to increase the size of an image of an object B. Resolution – how clear the image appears…the more magnified, the more blurry the image (light bends) C. Types of Microscopes
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The Cell Honors Biology Chapter 4 • Microscopes • A. Magnification – to increase the size of an image of an object • B. Resolution – how clear the image appears…the more magnified, the more blurry the image (light bends) • C. Types of Microscopes • 1. Light microscope – light shines through the object • a. simple – one lens • b. compound – two lenses (what we have) • 2. Electron microscope – bounces a beam of electrons off of an object coated in gold. Only see surfaces of objects
D. Parts and Function of a compound light microscope 1. Base – supports microscope 2. Light – shines through the object 3. Stage – where the slide sits when viewing 4. Eyepiece lens – lens closest to eye (mag.10X) 5. Arm – connects the eyepiece to the base 6. Objective lens – lens closest to object a. 4X b. 10X c. 40X d. 100X 7. Diaphragm – adjusts the amount of light shining through the object (disk vs. iris) 8. Nosepiece – rotates the objective lenses 9. Coarse adjustment – brings object into the field of view 10. Fine adjustment – focuses object, increases resolution
Eyepiece Arm Coarse Adjustment Fine Adjustment Nosepiece Objective lens Stage Diaphragm Light Base