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Synthetic Biology: Design and characterization of antiswitches in E. coli .

Synthetic Biology: Design and characterization of antiswitches in E. coli . Matt Gemberling Davidson College 25 March, 2006. Outline for presentation. Background on Antiswitches Antiswitches and how they function Results in Prokaryotes Conclusions. Background.

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Synthetic Biology: Design and characterization of antiswitches in E. coli .

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  1. Synthetic Biology: Design and characterization of antiswitches in E. coli. Matt Gemberling Davidson College 25 March, 2006

  2. Outline for presentation • Background on Antiswitches • Antiswitches and how they function • Results in Prokaryotes • Conclusions

  3. Background • Smolke and Bayer (2005) created antiswitches in Yeast (S. cerevisiae) • “On” and “Off” switches were designed • RNA-mediated vs. Protein regulated translation

  4. Antiswitches

  5. Aptamer - nucleic acid (e.g. RNA) that binds a ligand with high specificity Caffeine Theophylline Theophylline aptamer

  6. Ribozymes • Ribozymes are sequences of auto-catalytically cleaving RNA • Ribozymes provide mechanism for generating Antiswitch RNA

  7. Results

  8. Conclusions • Current antiswitch designs are non-functional in E. coli • Future work • Express our antiswitches in yeast • Test for ribozyme cleavage in E. coli

  9. Acknowledgements • Collaborators • O. Hernandez, T. Odle, K. DeCelle, N. Cain, A. Drysdale, M. Cowell, J. Ryan, F. Trappey, Dr. A. M Campbell, Davidson College • D. Endy and R. Rettberg, MIT • T. Bayer, CalTech • Financial support from HHMI, MIT, and Davidson Biology Dept.

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