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P-Src T-Src. Forskolin (10 M) 0 1 5 10 20 30. min.
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P-Src T-Src Forskolin (10 M) 0 1 5 10 20 30 min Forskolin induces Src (tyr 416) phosphorylation in LX-2 cells. LX-2 cells were cultured in serum-free DMED containing stellate cell growth supplement and treated with 10 M forskolin. Cells were lysed in indicated time points. Phosphorylated and total Src were expressed in the cells as detected by immunoblot analysis using the phosphorylated and total Src antibodies. Supplemental Figure 1
P-ERK ½ T-ERK 1/2 Forskolin (10 M) 0 1 5 10 20 30 min Forskolin induces ERK1/2 phosphorylation in LX-2 cells. LX-2 cells were cultured in serum-free DMED containing stellate cell growth supplement and treated with 10 M forskolin. Cells were lysed in indicated time points. Phosphorylated and total ERK1/2 were expressed in the cells as detected by immunoblot analysis using the phosphorylated and total ERK1/2 antibodies. Supplemental Figure 2
