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Studies towards stereoselective bionanocatalysis on gold nanoparticles

Studies towards stereoselective bionanocatalysis on gold nanoparticles. Ryszard Ostaszewski ,  Hanna Hibner  Instytut Chemii Organicznej Polskiej Akademii Nauk (ICHO PAN), Kasprzaka 44/52, Warszawa 01-224, Poland. Applications of enzymes in nanotechnology. Biosensors

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Studies towards stereoselective bionanocatalysis on gold nanoparticles

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  1. Studies towards stereoselective bionanocatalysis on gold nanoparticles Ryszard Ostaszewski,  Hanna Hibner  Instytut Chemii Organicznej Polskiej Akademii Nauk (ICHO PAN), Kasprzaka 44/52, Warszawa 01-224, Poland

  2. Applications of enzymes in nanotechnology • Biosensors Du D., Chen Sh., Cai J., Zhang A., Biosens. Bioelectron., 2007, 23, 130-134 • Immunoenzymatic tests • Biocatalysis

  3. Biocatalysis Phadtare S., Vinod V.P., Mukhopadhyay K., Kumar A., Rao M., Chaudhari R.V., Sastry M.,Biotechnology and Bioengineering, 2004, 85 (6), 629-637

  4. Biocatalysis a – free enzyme b – glucose oxidase on AuNP Pandey P., Singh S.P., Arya S.K., Gupta V., Datta M., Singh S., Malhotra B.D., Langmuir, 2007, 23, 3333-3337

  5. Enzymatic kinetic resolution – model reaction Ps.cepacia lipase E = 37 Boaz N.W., J. Org. Chem., 1992, 57, 4289-4292 Ultrasounds + Ps.cepacia lipase E = 458 Ribeiro C.M.R., Passaroto E.N., Brenelli E.C.S., Tetrahedron Lett., 2001, 42, 6477-6479

  6. Native enzymes with and without nanoparticles

  7. Enzymes covalently immobilized on nanoparticles • Linker: α-lipoic acid • Coupling agent: 1,1’-carbonyldiimidazole • Modified procedure from: Pandey P., Singh S.P., Arya S.K., Gupta V., Datta M., Singh S., Malhotra B.D., Langmuir, 2007, 23, 3333-3337

  8. Pseudomonas cepacia lipase • About 10% of the enzyme added was immobilized on nanoparticles • The same quantity of the native enzyme gives the same conversion

  9. Pseudomonas cepacia lipase

  10. Other enzymes

  11. Conclusions • Various enzymes were successfully immobilized on gold nanoparticles. • Obtained bionanocatalysts were active and catalysed model reaction similarly to native enzymes. • Immobilized enzymes can be used in a few catalytic cycles.

  12. Acknowledgments: This work was supported by project ”Biotransformations for pharmaceutical and cosmetics industry” No. POIG.01.03.01-00-158/09-01 part-financed by the European Union within the European Regional Development Fund.

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