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Activity #2: Ethanol and Tobacco Toxicity in Living Animal Cells

Activity #2: Ethanol and Tobacco Toxicity in Living Animal Cells. Step 1. Infusion of hay, dry grass, or pond mud Opacity Check: try reading a newspaper through the culture jar to check for cloudiness. Infusion. Step 2 - Treatments. Add various substances to other culture tubes

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Activity #2: Ethanol and Tobacco Toxicity in Living Animal Cells

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  1. Activity #2: Ethanol and Tobacco Toxicity in Living Animal Cells

  2. Step 1 • Infusion of hay, dry grass, or pond mud • Opacity Check: try reading a newspaper through the culture jar to check for cloudiness Infusion

  3. Step 2 - Treatments • Add various substances to other culture tubes • Water (leaves one tube untreated as a control) • Alcohol, tobacco • Here we also tested mouthwash and toothpaste

  4. Step 3a – Examine Tubes Visually Water (control) Mouthwash Toothpaste Coca Cola Tobacco Ethanol Observe and Note Immediate Effects (Repeat again after two days)

  5. Step 3b Inspect the Organisms Examine Organisms Under the Microscope – Initially and Two Days Later

  6. Step 4. Effects of Treatment Over Time As seen under a microscope, number and type of protists should change rapidly – in a matter of hours

  7. Step 5 DNA Extraction Water (control) Coca Cola Mouthwash Toothpaste Tobacco Ethanol DNA

  8. Water (control) DNA Mainly Salt

  9. Ethanol Mainly Salt

  10. Tobacco DNA Salt

  11. Mouthwash DNA

  12. Toothpaste DNA

  13. Coca Cola DNA

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