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Ryan Butryn and Rick Naro. Phytoplankton Diversity in New York Lakes. http://www.turnerdesigns.com/t2/instruments/10au.html. http://www.durr.demon.co.uk/volvox.html. Study Sites. Analysis of phytoplankton diversity and chlorophyll-a levels in New York lakes: Arbutus Lake Wolf Lake
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Ryan Butryn and Rick Naro Phytoplankton Diversity in New York Lakes http://www.turnerdesigns.com/t2/instruments/10au.html http://www.durr.demon.co.uk/volvox.html
Study Sites • Analysis of phytoplankton diversity and chlorophyll-a levels in New York lakes: • Arbutus Lake • Wolf Lake • Catlin Lake • Oneida Lake • Green Lake • Onondaga Lake • Skaneateles Lake Wolf Lake
Chlorophyll Study Site Procedures • Collect water sample from defined lake layers • epilimnion • metalimnion • hypolimnion • Green Lake sampling included • chemocline • monimolimnion Arbutus Lake
Chlorophyll Lab Procedure • Turner Designs 10-AU Field Fluorometer • Measures the fluorescence of chlorophyll-a • Highly sensitive • Suitable for extractive and in vivo analysis • Sample Processing • 100ml water sample filtered • Filter paper dissolved in 10ml acetone http://www.turnerdesigns.com/t2/instruments/10au.html
A Special Case: Green Lake • Lake profile appears to be similar to other low productivity lakes • Highest chlorophyll-a level is found in the chemocline
Why is chlorophyll-a so high? • We hypothesize that green photosynthetic bacteria should be present in solution and masked by the purple bacteria • Further processing is required to confirm this.
Phytoplankton Collection • Van Dorn trap used to sample each lake layer • Samples stabilized with preservative solution • Identified and counted under microscope
Phytoplankton Divisions Quadricoccus euryhalinicus
Summary of Observations • Chlorophyll • Highest in epilimnion and hypolimnion in eutrophic lakes, lower in metalimnion • Relatively constant density in oligotrophic lakes • Green Lake a special case • Phytoplankton • Increasing eutrophication = higher phytoplankton diversity • Cyanophytes tend to dominate eutrophic lakes • Chlorophytes dominate more oligotrophic lakes • More plankton diversity in epilimnion, more plankton numbers in metalimnion and hypolimnion