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Chem-805/806. Structure determination. Strategies to assign resonances. Entry point: Try to find all the resonances that can be assign without ambiguity from 1D-NMR Predict chemical shifts (H and C) and compare with observed shifts.
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Chem-805/806 Structure determination
Strategies to assign resonances • Entry point: Try to find all the resonances that can be assign without ambiguity from 1D-NMR • Predict chemical shifts (H and C) and compare with observed shifts. • Integration can be used to match number of protons in overlapping multiplets • Predict multiplets and see how they match
Bookkeeping • Determine which signals belong to CH, CH2 and CH3. Integrate H-NMR • Create tables of proton and carbon shifts related to each other • Try to understand the shift (which type of oxygen do you have (carbonyl, ester, ether..) • Build substructure that make sense of multiplicity. • Propose a structure. Number the structure (UPAC rules). Assign H and C NMR.
Example : H-NMRC8H15NO2 3H 2H + 1H 2H 1H 1H 1H 1H 1H 1H 1H
C8H15NO2 Example COSY 3H, t 2H, q CH3 - CH2 - O
C8H15NO2 Example HMQC CH2 CH2 CH2 CH2 O-CH2 CH3 CH OH? NH?
Example : H-NMRC8H15NO2 In C13 NMR: 170 ppm ester 3H (CH3-CH2-O-C=O) 2H + 1H 2H OH or NH 1H 1H 1H 1H 1H 1H 1H
C8H15NO2 Example COSY 3H, t CH3-CH2-O-CO- NH 2H, q All atoms are accounted for CH2 CH2 CH CH2 CH2 NH Deshielded by N
Example Jeffrey H. Simpson, “Orgamic structure determination using 2-D NMR spectroscopy”
Example : H-NMRC8H15NO2 3H 2H + 1H 2H 5 NH 6’ 4’ 2’ 3 6 5’ 2 4
Assignment of quaternary Carbons Quaternary carbons: 176.3, 154.8, 143.4, 125.2, 120.4 C4 = 176 C10 = 155, most deshielded due to oxygen C11 C10 C4
HMBC 2 12 13 6 3 10 4 13 12 5 7 2 11
Strategy for Unknown molecules • Consider solvent used: contribution to H and C NMR • Inspect H and C spectra for obvious clues Deshielded HOD Aromatic Solvent: D2O CHO
EXPANSIONS CH – O Aromatic Ortho CHO
C13 O CH-O Aromatic CH O 193.3 ppm CH=O Not shown
Unknown: HMQC • CHO group: 10.2 ppm – 193.3 • 4 aromatic H: disubst., Ortho (2D, 2T) • Deshielded H at 5.2, coupled to C 100.1 ppm : deshielded by 2 O? CH-O O CH O Ortho aromatic CHO
Unknown: HMQC expansion CHO CH2-O 4 x CH-O