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This gel depicts the NEB 1kb ladder and a digestion of the plasmid pIS1-Eef25UTR-TOPmut-renilla with the following enzymes : NcoI , NheI , Ncol -HF + Nhel -HF. Plasmid 38236 pIS1-Eef25UTR-TOPmut-renilla. Ncol -HF + Nhel HF. 1kb Ladder. Ncol -HF. Nhel -HF. Uncut.

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  1. This gel depicts the NEB 1kb ladder and a digestion of the plasmid pIS1-Eef25UTR-TOPmut-renillawith the following enzymes: NcoI, NheI, Ncol-HF + Nhel-HF Plasmid 38236 pIS1-Eef25UTR-TOPmut-renilla Ncol-HF +Nhel HF 1kb Ladder Ncol-HF Nhel-HF Uncut 0.5 1 1.5 2 3 4 5 6 8 10 DNA digested withNcoI, NheI, Ncol-HF+Nhel-HF for 2hours Gel: 0.8% TAE stained with 100 ml 1X TAE and 5uL of ethidium bromide Ran for2 hours at 60 volts

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