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K Ras Unusual results

K Ras Unusual results. Linda Meredith 29 th Sept 2009. Mutation calling criteria. Criteria for calling a mutation positive is the fall in one peak usually the GG peak and increase in another peak. GG peaks often compared against each other for reference.

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K Ras Unusual results

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  1. K Ras Unusual results Linda Meredith 29th Sept 2009

  2. Mutation calling criteria • Criteria for calling a mutation positive is the fall in one peak usually the GG peak and increase in another peak. • GG peaks often compared against each other for reference

  3. Complex rearrangement Codon 12/13 c.36T>A [Gly12Gly] 9M1460 c.38G>C [Gly13Ala] c.40G>T?? Mutant called G>A?? A↑ T↓ G↓ C↑ G↓ A↓ Normal

  4. Two mutations in 1 sample 9M4761 Both GG peaks decreased Confirmed by Real time PCR DCX kit Normal

  5. Unusual mutation c.159G>T p.59 9M3983 Mutation G↓ T↑ Normal

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