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Revision – Concept map. Human Genome Project. Procedures Genetic mapping Physical mapping DNA sequencing Techniques Use of microsatellites (STRs) Restriction enzyme mapping Sanger method PCR Gel electrophoresis. Human Genome project. Terminology Restriction mapping Primer
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Human Genome Project • Procedures • Genetic mapping • Physical mapping • DNA sequencing • Techniques • Use of microsatellites (STRs) • Restriction enzyme mapping • Sanger method • PCR • Gel electrophoresis
Human Genome project • Terminology • Restriction mapping • Primer • Microsatellite / Short Tandem Repeat • Anneal • Genetic marker • Hybridise
Detecting Genetic Disorders • Procedures • Pedigree analysis • Linkage analysis (both genetic and physical) • Screening • Techniques • PCR • Chromosome walking • Sanger method • Gel electrophoresis
Detecting genetic disorders • Terminology • Probe • Genomic library • Chromosome walking • Hybridise
DNA profiling • Procedures • DNA isolation • Restriction enzyme digestion • Separation of VNTR • Fixing pattern on filter • Techniques • Restriction enzyme digestion • Gel electrophoresis • Southern blotting
DNA profiling • Terminology • Minisatellite / Variable Number Tandem Repeat • Probe • Hybridise