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Revision – Concept map

Explore the procedures and techniques involved in genetic mapping for the Human Genome Project, detecting genetic disorders, and DNA profiling. Learn about concepts such as physical mapping, DNA sequencing, gel electrophoresis, and more.

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Revision – Concept map

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  1. Revision – Concept map

  2. Human Genome Project • Procedures • Genetic mapping • Physical mapping • DNA sequencing • Techniques • Use of microsatellites (STRs) • Restriction enzyme mapping • Sanger method • PCR • Gel electrophoresis

  3. Human Genome project • Terminology • Restriction mapping • Primer • Microsatellite / Short Tandem Repeat • Anneal • Genetic marker • Hybridise

  4. Detecting Genetic Disorders • Procedures • Pedigree analysis • Linkage analysis (both genetic and physical) • Screening • Techniques • PCR • Chromosome walking • Sanger method • Gel electrophoresis

  5. Detecting genetic disorders • Terminology • Probe • Genomic library • Chromosome walking • Hybridise

  6. DNA profiling • Procedures • DNA isolation • Restriction enzyme digestion • Separation of VNTR • Fixing pattern on filter • Techniques • Restriction enzyme digestion • Gel electrophoresis • Southern blotting

  7. DNA profiling • Terminology • Minisatellite / Variable Number Tandem Repeat • Probe • Hybridise

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