（ A ）

1. Supplement fig. 3 Effects of ActivinA on chemotaxis, migration, and proliferation of HPDLC-3O and -3Q. （A） ** ** ** Cell number Chemotaxis number of cells (Cells/field) 0h 0h ActivinA ActivinA (ng/ml) (ng/ml) ActivinA 0 0 (ng/ml) (ng/ml) 0 ** 24h 48h ** ActivinA ActivinA 100 10 10 100 0 0 10 100 (ng/ml) (ng/ml) 0 10 100 ActivinA （C） Chemotaxis number of cells (Cells/field) OD450nm Supplemental Fig. 3 Effects of Activin A on chemotaxis, migration, and proliferation of HPDLC-3O and -3Q. (A) In chemotactic assay, HPDLC-3O, derived from a premolar of 20-year-old female (upper) and -3Q, derived from a premolar of a 21-year-old female (bottom) was seeded on cell inserts with porous membranes. Assay was performed as described in Materials and Methods (n = 4). **P < 0.01. Bars = 100 mm. (B) Migratory activity of HPDLC-3O (upper) and -3Q (bottom) was analyzed by using a scratch wound healing assay. Assay was performed as described in Materials and Methods. Results are representative of three separate experiments. **P < 0.01. Bar = 100 m. (C) Proliferation assay of HPDLC-3O (left) and -3Q (right) cultured in 10%FBS/MEM plus Activin A (0, 10, or 100 ng/ml) was performed using a WST-1 assay kit. On Day 0, 3, 5 of culture, proliferating cells were measured at absorbance of 450 nm. Data were described graphically. Values are the means ± SD from three independent experiments. Experiments were performed in duplicate. *P < 0.05, **P < 0.01. These results support the findings presented in Fig. 3. * ActivinA ** * OD450nm Cell number ActivinA 100 100 ActivinA ActivinA (ng/ml) (ng/ml) （B）