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ПЕРСПЕКТИВА РАЗРАБОТКИ КУЛЬТУРАЛЬНОЙ ИНАКТИВИРОВАННОЙ ВАКЦИНЫ ПРОТИВ ГЕПАТИТА С

This article explores the cultural inactivation of Hepatitis C vaccine to prevent the infection. It discusses the basic parameters of HCV infection, HCV-specific antigens, replication inhibition, and neutralizing antibodies. Additionally, it examines the antiviral activity of α-Interferon and the identification of cytopathogenic HCV isolated from infected mice. The study also looks at the ability of inactivated HCV-containing material to induce HCV-specific antibodies in mice.

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ПЕРСПЕКТИВА РАЗРАБОТКИ КУЛЬТУРАЛЬНОЙ ИНАКТИВИРОВАННОЙ ВАКЦИНЫ ПРОТИВ ГЕПАТИТА С

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  1. ПЕРСПЕКТИВА РАЗРАБОТКИ КУЛЬТУРАЛЬНОЙ ИНАКТИВИРОВАННОЙ ВАКЦИНЫ ПРОТИВ ГЕПАТИТА С П.Г.ДЕРЯБИН, Е.И. ИСАЕВА, A.C. CУХНО, Д.К. ЛЬВОВ НИИ ВИРУСОЛОГИИ ИМ. Д.И.ИВАНОВСКОГО РАМН , МОСКВА

  2. Hepatitis C specific prevention BASIC PARAMETERS OF INFECTION INDUCED BY ISOLATED HCV VARIANT IN PRIMARY HUMAN ENDOTELIAL CELLS %OFCELLS lg TCD50 HCV RNA + HCV RNA + INMONOLAYER 8 100 7 80 6 5 60 4 40 3 2 20 1 1 2 3 4 5 6 7 8 9 TIME AFTER CELL INFECTION BY HCV (DAYS)

  3. Hepatitis C specific prevention HCV SPECIFIC ANTIGENS IN HCV INFECTED CHICK EMBRYO FIBROBLASTS DETECTED BY INDIRECT IMMUNE FLUORESCENCE METHOD USING MONOCLONAL ANTIBODIES TO RECOMBINANT HCV CORE PROTEIN.

  4. Hepatitis C specific prevention HCV REPLICATION IN VITRO IS INHIBITED BY HUMAN ANTI-HCV NEUTRALIZING ANTIBODIES a) RT PCR Before After neutralization neutralization tenfold virus dilutions used b) HCV CYTOPATHOGENIC EFFECT

  5. Hepatitis C specific prevention α- INTERFERON ANTIVIRAL ACTIVITY STUDYIN SW-13 CELLCULTURES INFECTED WITH HEPATITIS C VIRUS lg ТCD 50 Contol HCV infected cells Interferon ffter virus absorbtion Interferon 24 h p.i. Interferon 24 h and 48 h p.i. 4th day post infection 5th day p.i. 6th day p.i. Time after HCV infection of SW-13 cell cultures

  6. Hepatitis C specific prevention INFECTION OF RABBITS INDUCED BY CYTOPATHOGENIC HCV VARIANT (STUDY OF BLOOD SERA SAMPLES COLLECTED ON 24-th DAY POSTINFECTION )

  7. Hepatitis C specific prevention HCV infected mouse liver (56 days after virus administrationby intravenously way) a) liver of uninfected mouse • b) liver of HCV infected mouse

  8. Hepatitis C specific prevention Liver of uninfected mouse. Liver has normal structure. A central vena can noticed. Normal hepatocytes. Zoom x 100 Liver of HCV infected mouse. (56 day after the infection) Large periportal cell infiltration. Zoom x 400

  9. Hepatitis C specific prevention HCV SPECIFIC ANTIBODIES TO DIFFERENT HCV SPECIFIC PROTEINS DETECTED BY WESTERN BLOT (RecomBlot HCV IgG) IN MOUSE BLOOD SERUM SAMPLES r Core (aa 1- aa 115) r NS 3 (aa 1007 – aa 1534) r NS 4 (aa 1616 – aa 1862) r NS – 4 –1 (aa 1680 – aa 1796)

  10. Hepatitis C specific prevention Identification of cytopathogenic HCV isolated from infected mice

  11. Hepatitis C specific prevention AN ABILYTY OF INACTIVATED HCV CONTAINING MATERIAL TO INDUCE HCV SPECIFIC ANTIBODIES IN MICE

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