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Coffalyser MLPA data quick analysis PPT

Coffalyser controls. . . . . . . RemoveA. AddA. ComboboxA. PlayA. ComboboxB. PlayB. . . MLPA mix Selector combobox. LOAD BUTTON. In this Manual there will be referred to the following buttons! Note that buttons or text that you may need to select in the Coffalyser are set Italic.. Coffalyser start userform.

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Coffalyser MLPA data quick analysis PPT

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    1. Coffalyser MLPA data quick analysis PPT FILTERED DATA

    2. Coffalyser controls

    3. Coffalyser start userform

    4. Select your probe mix (double click)

    5. Press play next to load

    6. Start a new project (include all samples in a experiment)

    7. Import filtered data (reference)

    8. Import filtered data (samples)

    9. Re-open the Coffalyser

    10. Quality test 2

    11. Quality test 2

    12. Choosing your analysis method Coffalyser method Coffalyser method will work for most MLPA mixes and is probably the most robust method. Recommended for tumor samples Control probe method Control probe method is recommended for cytogenetic diseases and any MLPA mix containing control probes which can be trusted for the samples that you are using Population method Population method, normalizes the MLPA data against all probes in the mix and should be used when a MLPA mix doesnt contain any control probes and not many probes in a mix are expected to change (p036, p070, MRX) Methylation status analysis Methylatin status analysis can only be performed for the ME kits, NOTE:! UNCUT sample should be imported as references, CUT samples should be imported as samples. Both CUT and UNCUT should have the same names except for the end part e.g. sample01-cut and sample01-uncut. Click on PLAYB to start the analysis! DO NOT USE _ or ^ in your sample names! USE UNIQUE run names! (not sample1, sample12 but sample01 and sample 12!!

    13. No reference run

    14. Storing the project summary

    15. Exploring analyzed results

    16. Exploring sample graph

    17. Sample report exploration

    19. Results exploration through sheets

    20. Reference run results

    22. Advanced Settings

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