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Current Proteome Profiling Methods and Applications

Current Proteome Profiling Methods and Applications. Yetrib Hathout, Ph.D. Center for Genetic Medicine Children’s National Medical Center & The George Washington University yhathout@cnmcresearch.org. The goal on many proteomic studies is determining protein ratios between samples

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Current Proteome Profiling Methods and Applications

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  1. Current Proteome Profiling Methods and Applications Yetrib Hathout, Ph.D. Center for Genetic Medicine Children’s National Medical Center & The George Washington University yhathout@cnmcresearch.org

  2. The goal on many proteomic studies is determining protein ratios between samples representing different biological states. diseased tissue  healthy tissue drug treated  non-tread drug resistant  non-resistant

  3. Current Methods Mass spectrometry Reudi Aebersold and Matthias Mann. Nature 2003, 422:198-207

  4. Unlabeld Cells sample 1 regular Arg and Lys Mitochondrial fraction Nuclear fraction Mix cells1:1 ER fraction Labeled Cells sample 2 13C6-Arg & 13C6,15N2-Lys Extract proteins And Digest with tryspin LC-MS and MS/MS analysis Zoom scans Based peak Quantification & Validation MS/MS Database search & protein identification

  5. Applications • Understanding cellular and molecular mechanisms of age related macular degeneration. • ER stress response and protein trafficking in Vanishing White Matter-Disease. • Defining biomarkers and therapeutic targets in pediatric brain tumors.

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