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Immunoassay Research at the UW Department of Bioengineering

Immunoassay Research at the UW Department of Bioengineering. By Anton Leitner. William H. Foege Building. Department of Genome Sciences Department of Bioengineering Bhushan Toley – PhD, Senior Research F ellow interested in diagnostic devices, microfluidics , engineering.

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Immunoassay Research at the UW Department of Bioengineering

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  1. Immunoassay Research at the UW Department of Bioengineering By Anton Leitner

  2. William H. Foege Building • Department of Genome Sciences • Department of Bioengineering • BhushanToley – PhD, Senior Research Fellow interested in diagnostic devices, microfluidics, engineering. • Dr. Barry Lutz - Research Assistant Professor interested in biosensor chemical transport & kinetics, sound-based microfluidic systems, point-of-care diagnostics

  3. Goals for the quarter • Learn lab procedures and how to operate equipment • Construct a calibration curve • Gather kinetic data for protein flow rate • Construct binding curve • Interpret data

  4. Calibration Curve Data

  5. Calibration Curve http://logicalgenetics.com/forums/viewtopic.php?t=636

  6. Immunoassay Basics Nano-sized fluorescent bead Protein http://www.thedailysheeple.com/american-fertility-rates-drop-because-of-global-population-stabilization-agenda_102012/pregnancy-test-1 Antigen Substrate If too much protein is binding, you have the flu

  7. NitrocelluloseDetection Strip http://www.bio-rad.com/prd/en/US/LSR/PDP/9b5b2069-356c-447b-a0fd-cae196945394/Nitrocellulose-Membrane,-0.45-%C2%B5m

  8. Intensity vs Flow Rate

  9. Interpreting The Data

  10. Thanks to: • BhushanToley • Barry Lutz

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