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Small Angle X-ray Scattering

Small Angle X-ray Scattering. Rob Lewis Centre for X-ray Physics and Imaging School of Physics and Materials Engineering Monash University. Bragg’s Law. 2d sin θ = n λ or d = n λ /2sin θ Small angles (< 5 o ) give information on large structures – 1 to 100nm. . d. SAXS.

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Small Angle X-ray Scattering

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  1. Small Angle X-ray Scattering Rob Lewis Centre for X-ray Physics and Imaging School of Physics and Materials Engineering Monash University

  2. Bragg’sLaw 2d sin θ= nλor d = nλ/2sin θ Small angles (< 5o ) give information on large structures – 1 to 100nm  d

  3. SAXS Crystallography Microstructure Structure 10-11m 10-9 10-7 10-5 Elliot Gilbert ANSTO

  4. Diffraction Configuration

  5. SAXS • Works on live wet specimens • Time resolution - sub microsecond • Two main variants • Solution scattering • Protein folding • Protein ligand interactons • Fibre diffraction • Tissue analysis • Muscle operation

  6. Diffraction

  7. Diffraction

  8. 6 models of sAPPα695 • Dark indicates areas of superposition • A B and C are orthogonal views Crystal model of N terminal domain of APP Gralle et al Biophys J 83, 3513 (2002)

  9. Collagen Axial Structure Overlap Overlap Overlap Gap Gap Gap Tropocollagen Secreted from cell 1.5nm N 67nm Proteolytic cleavage Procollagen N protease Procollagen C protease 10.4nm 300nm Cross links involve lysyl oxidase C

  10. Collagen Rat tail tendon Camera length: 9.35m Wavelength: 0.154nm

  11. Different tissues

  12. Diffracted Intensity: Trend

  13. Osteochondral Autograft Transplantation OATS

  14. OATS surgical technique MARIARC MRI, University Liverpool, UK Incorrect graft harvest and recipient socket angles, are the most common OAT surgical errors and reasons for failure.

  15. Slide: S Arnotzky

  16. Lamb Cartilage Study

  17. Myelin Diffraction

  18. Muscle diffraction Resting Contracted

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