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I Fórum em Neurobiologia do Estresse

I Fórum em Neurobiologia do Estresse. Neural circuitry of fear and pain and their possible relationships with anxiety and panic. Jesús Landeira-Fernandez PUC-Rio & UNESA. Functions of the Nervous System. Two functions of the nervous system.

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I Fórum em Neurobiologia do Estresse

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  1. I Fórum em Neurobiologia do Estresse Neural circuitry of fear and pain and their possible relationships with anxiety and panic Jesús Landeira-Fernandez PUC-Rio & UNESA

  2. Functions of the Nervous System Two functions of the nervous system Regulation of the internal environment through homeostasis Visceral Nervous System Interact with external environment through behavior Somatic Nervous System

  3. Organization of the Nervous System STIMULUS BEHAVIOR Defense Feeding Reproduction

  4. Organization of the Defense System Dangerous Stimulus Nervous System Defensive Response

  5. Organization of the Defense System Dangerous Stimulus Innate Cause some threat to the life to the individual Learned

  6. Organization of the Defense System

  7. Organization of the Defense System Danger Stimulus Behavioral Inhibition Freezing Escape Defensive Response Fight Behavioral Activation ACTIVITY BURST

  8. Organization of the Defense System Theoretical Constructs

  9. Organization of the Fear Motivational System Somatic Nervous System Visceral Nervous System

  10. Organization of the Pain Motivational System Somatic Nervous System Visceral Nervous System

  11. Interaction between the Pain and the Fear Motivational System Pain motivational system activates fear motivational system through classical conditioning Pain Motivational System Fear Motivational System Contextual Fear Conditioning Neutral Stimuli

  12. Interaction between the Fear and Pain Motivational System Fear motivational system inhibits pain motivational system through analgesia Fear Motivational System Pain Motivational System Analgesia

  13. Interaction between the Fear and the Pain Motivational System Classical Conditioning Analgesia

  14. Contextual Fear Conditioning Paradigm First Day: Training Session 0:00 3:00 3:20 3:40 4:00 A few minutes of habituation Dia 1 Treinamento A few rapid and not very intense footshocks 0:00 3:00 Second Day: Testing Session

  15. Typical Contextual fear Conditioning Result Freezing depends on the intensity of the shock

  16. Activation of the Pain Motivational System in the Vacuum: The Immediate Shock Deficit

  17. Activation of the fear Motivational System in the Vacuum: The Immediate Shock Deficit

  18. Delayed but not Immediate Shock Induces Analgesia

  19. Immediate Shock does not Induce Analgesia

  20. Opioid Analgesia

  21. Opioid Analgesia

  22. Non-Opioid Analgesia

  23. Fear and Pain Motivational Systems Dangerous Stimuli Motivational Systems Defense Responses Distal Innate Fear Freezing Learned Innate Classical Conditioning Analgesia Pain Activity Burst Proximal

  24. Fear and Pain x Anxiety and Panic Motivational Systems Defense Responses Motivational Systems Dysfunctions Fear Anxiety Freezing Classical Conditioning Analgesia Panic Attack Pain Activity Burst

  25. Deakin-Graeff Model Dysfunctions Anxiety Activates Inhibits Panic Attack

  26. Freud and the anxiety disorders _______________

  27. Function x Dysfunction Fanselow Model Deakin-Graeff Model Functions Dysfunctions Fear Anxiety Activates Classical Conditioning Inhibits Analgesia Panic Attack Pain

  28. Animal Models to Investigate Anxiety and Panic Attack Contextual fear conditioning (Model of Generalized Anxiety Disorder) dPAG Electrical Stimulation (Model of Panic Attack)

  29. Animal Model of Panic Attack dPAG electrical stimulation

  30. dPAG electrical stimulation Increasing the intensity of the dPAG electrical stimulation there is first a freezing response followed by vigorous running and jump reactions.

  31. The Periaqueductal Gray Matter Periaqueductal Gray (PAG) Electrode Tract Stimulation Site in the Dorsal Portion of the PAG Cerebral Aqueduct

  32. Does Anxiety (contextual fear conditioning) Inhibits Panic Attacks (dPAG electrical stimulation)? dPAG Electrical Stimulation (Model of Panic Attack) Contextual fear conditioning (Model of Generalized Anxiety Disorder)

  33. Does Anxiety (contextual fear conditioning) Inhibits Panic Attacks (dPAG electrical stimulation)?

  34. Does Anxiety (contextual fear conditioning) Inhibits Panic Attacks (dPAG stimulation- NMDA 15.0µg/µl)?

  35. Does Anxiety (PTZ - 15.0 mg/kg i.p) Inhibits Panic Attacks (dPAG stimulation- NMDA 15.0µg/µl)?

  36. Two Types of Freezing: the context shift test Contextual fear conditioning

  37. Two Types of Freezing: the context shift test Contextual fear conditioning

  38. Two Types of Freezing: the context shift test dPAG electrical stimulation

  39. Two Types of Freezing: the context shift test dPAG electrical stimulation

  40. Two Types of Freezing: the context shift test: sumary Contextual fear conditioning dPAG electrical stimulation

  41. Two Types of Freezing Freezing Induced by Contextual Fear Conditining Amygdala vPAG Freezing Freezing Induced by dPAG Electrical Stimulation ? dPAG ?

  42. Comparison between dlPAG and vlPAG electrical stimulation

  43. Comparison between dlPAG and vlPAG electrical stimulation Freezing after dPAG electrical stimulation

  44. Effect of vPAG Lesions on Conditioned and Unconditioned Freezing

  45. Histology of vPAG Lesion and dPAG Electrical Stimulation vPAG Lesions dPAG Electrical Stimulation

  46. Effect of vPAG Lesions on Conditioned and Unconditioned Freezing Contextual fear conditioning dPAG electrical stimulation

  47. Effect of Amydgala Lesions on Conditioned and Unconditioned Freezing

  48. Histology of Amygdala Lesion and dPAG Electrical Stimulation dPAG Stimulation Amygdala Lesions

  49. Effect of Amygdala Lesions on Conditioned and Unconditioned Freezing Contextual fear conditioning dPAG electrical stimulation

  50. So far, these are the results … vPAG

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