1 / 1

Using Your Staining Antibodies with BD CompBeads

Using Your Staining Antibodies with BD CompBeads. Verify Ab has Kappa light chain. Match Ab to species. Mouse - use BD#552843. Rat - use BD#552844. Rat/Hamster - use BD#552845. Single stain beads as if 1x10 6 cells. Use amount to match or exceed brightness of stained cells.

emily
Download Presentation

Using Your Staining Antibodies with BD CompBeads

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Using Your Staining Antibodies with BD CompBeads Verify Ab has Kappa light chain Match Ab to species Mouse - use BD#552843 Rat - use BD#552844 Rat/Hamster - use BD#552845 Single stain beads as if 1x106 cells Use amount to match or exceed brightness of stained cells Run as compensation sample Use validated instrument settings Fit beads on scatter plot Gate on singlet beads Acquire sufficient events Flow Core= 15,000 all M. Roederer= 50,000 gated Calculate compensation 060320 / EVC-FCC / Suzanne Mertens / saguila@emory.edu

More Related