Supplementary Figure S6. Effects of OHT and ectopic expression of FOXM1 and ERα in the

1. MDA-MB-231 A h OHT: 0 4 8 24 48 FOXM1 PLK CDC25b ERα Tubulin B C MDA-MB-231 MDA-MB-231 ERα: 0 µg 4 0 µg 2 6 FOXM1: 3 FOXM1 ERα ERα FOXM1 Cyclin D1 Cyclin D1 pS2 pS2 Tubulin Tubulin Supplementary Figure S6. Effects of OHT and ectopic expression of FOXM1 and ERα in the ERα-negative MDA-MB-231 breast carcinoma cell line. A) MDA-MB-231 cells were treated with 1 M OHT and cell lysates were prepared at the times indicated, and the expression of FOXM1, ERα, PLK, CDC25b and tubulin was analyzed by Western blotting. B and C) MDA-MB-231 cells were transfected with increasing amounts of FOXM1 and ERα. The expression of FOXM1, ERα, cyclin D1, pS2 and tubulin was analyzed by Western blotting. The results indicated that the expression of FOXM1 and ERα is not affected by OHT treatment. The results also showed that overexpression of ERα cannot induce the expression of FOXM1 and vice Versa. However, overexpression of FOXM1 can enhance the expression of cyclin D1. Millour et al Fig S6