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Lecture 2 An overview of Clinical Laboratory Testing Basic Clinical Chemistry

Purpose of Clinical Chemistry Tests . Measure levels of substances found normally in human blood that have biological functions. Examples: Glucose, CalciumDetect or measure non-functional metabolites or waste products. Examples: Creatinine, Blood Urea Nitrogen (BUN)Detect or measure substances

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Lecture 2 An overview of Clinical Laboratory Testing Basic Clinical Chemistry

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    1. Lecture 2 An overview of Clinical Laboratory Testing – Basic Clinical Chemistry

    2. Purpose of Clinical Chemistry Tests Measure levels of substances found normally in human blood that have biological functions. Examples: Glucose, Calcium Detect or measure non-functional metabolites or waste products. Examples: Creatinine, Blood Urea Nitrogen (BUN) Detect or measure substances that indicate cell damage or disease. Examples: Liver enzymes, such as ALT, Cardiac enzymes, such as CK-MB Detect or measure drugs or toxic substances. Examples: Dilantin, Drugs of abuse screen

    3. Types of Specimens for Chemical Analysis Whole blood, serum or plasma. The most common specimen is serum, collected in a tube with no anticoagulant so that the blood will clot. Urine – often 24 hour collections Others – Cerebrospinal Spinal Fluid (CSF) and other fluids

    4. Collection and Handling of Blood Specimens for Chemical Analysis Blood collection tubes for obtaining serum - Serum Separator Tubes (SST) do not have an anticoagulant but do contain a gel substance which will form an interface between the clot and the serum when the blood specimen is centrifuged. These tubes are sometimes referred to as “Tiger Tops”.

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