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Let’s Talk Science Action Project Fish Market Survey Results. Fish Market Survey. Tilapia. Red Snapper. $18.19/kg. $30.66/kg. Fall 2016. 8 different market names were targeted:. Cod Swordfish Sockeye Salmon King Salmon Snapper Red Snapper Atlantic Halibut Pacific Halibut.
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Fish Market Survey Tilapia Red Snapper $18.19/kg $30.66/kg
Fall 2016 8 different market names were targeted: • Cod • Swordfish • Sockeye Salmon • King Salmon • Snapper • Red Snapper • Atlantic Halibut • Pacific Halibut
Sampling • Fresh or frozen fish was purchased at local markets and grocery stores • Small tissue samples were taken by students • Placed into vials in LifeScanner kit • Shipped to LifeScanner and sequenced by partner lab - the Canadian Centre for DNA Barcoding, University of Guelph
Specimen DNA Barcoding Tissue Sample ExtractDNA Amplify DNA (PCR) Sequence DNA
DNA Barcoding Barcode sequences were then identified by comparing to the Barcode Of Life Data Systems: the global library for DNA Barcoding Oncorhynchus mykiss (Rainbow/Steelhead Trout)
DNA Barcoding Results were transmitted to the LifeScanner App + Oncorhynchus mykiss(Rainbow/Steelhead Trout)
Results Overview – Fall 2016 • 250 samples were analyzed • 226 yielded a DNA barcode sequence (90% success) • 16 specimens were removed from study due to errors (e.g., not including the market name) • 3 were removed as Insects were submitted instead of fish 207 total to study for mislabelling
Results Overview – Fall 2016 Of the 207 sequenced fish samples: • 12% (25 samples) were labelled with names not on the Canadian Food Inspection Agency Fish List • 8% (18 samples) were actually mislabelled fish 20% of the fish samples had labelling errors!
Results Overview – Fall 2016 43 Mislabelled Samples True mislabelling Name non-conformity 18 samples The scientific name and the declared common name exist on CFIA fish list but do not match 20 samples The declared common name does not exist on CFIA fish list 7 samples The scientific name does not exist on CFIA fish list 2 samples Neither the common name nor the scientific name exist on CFIA fish list
True mislabeling: Salmon • Example: BOLD-LX7 Market Label: Sockeye salmon DNA barcode match: Salmo salar(Atlantic Salmon or salmon) Atlantic Salmon (farmed): • Pollution depending on region farmed • Environmental impacts of farming practices • Health impacts • Economic impacts Male breeding Sockeye Salmon (Source: Public Domain, https://commons.wikimedia.org/w/index.php?curid=6248653 Atlantic Salmon (Source: Public Domain, https://commons.wikimedia.org/w/index.php?curid=19266403)
True mislabeling Other examples: • Substituting snapper with rosefish or salmon • Substituting cod with sole • Substituting basa with another type of catfish • Substituting sole with plaice • Substituting Pacific Cod with Atlantic Halibut • Substituting Atlantic Halibut with haddock Common Sole (Soleasolea) (Source: By Peter van der Sluijs - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=27120580)
Name non-conformity • Example: BOLD-H91 Market Label: Wild Sole DNA barcode match: Lepidopsettapolyxystra • This name is not on CFIA fish list but it is the name for Northern Rock Sole (still a type of sole) • Lepidopsettapolyxystra • (Orr & Matarese, 2000 ) • (Source: www.fishbase.ca)
Name non-conformity • Example: BOLD-EY9 Market label: Atlantic Cod DNA barcode match: Gadusmacrocephalus(Pacific Cod or cod). Source: www.fishwatch.gov (NOAA Fisheries Service) Still a type of cod, but labelled incorrectly
Name non-conformity Other examples of label issues: • Pacific Halibut versus Atlantic Halibut • Pacific Salmon versus Pink Salmon • Rockfish versus Pacific Rockfish • Sole versus Wild Sole Halibut (top) and salmon (bottom) (Source: By gran (self) (http://creativecommons.org/licenses/by/3.0, via Wikimedia Commons)
Thank you to all participating students and teachers! The project is ongoing – sign up now for the Spring 2017 Fish Market Survey on CurioCity https://explorecuriocity.org/Explore/ArticleId/4319/fish-market-survey.aspx
Supporters & Partners This project was made possible thanks to the following supporters & partners: