β-Amylase

1. 2020/12/29 β-Amylase - Creative Enzymes Products Enzymes for Research, Diagnostic and Industrial Use Services Home (/) /  β-Amylase β-Amylase Top (https://www.addtoany.com/share#url=https%3A%2F%2Fwww.creative- enzymes.com%2Fsimilar%2F-Amylase_754.html&title=%CE%B2- Amylase%20-%20Creative%20Enzymes) (/#facebook) (/#twitter) (/#linkedin) Official Full Name β-Amylase Background β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity. https://www.creative-enzymes.com/similar/-Amylase_754.html 1/6

2. 2020/12/29 β-Amylase - Creative Enzymes Synonyms saccharogen amylase; glycogenase; β amylase# β-amylase; 1#4-α-D-glucan maltohydrolase; EC 3.2.1.2; 9000-91-3 Research & Diagnostic Industry Catalog Product Name EC No. CAS No. Source Price CatalogEXWM-3884 ProductNameβ-amylase (/product/-amylase_14053.html) Services EC No.EC 3.2.1.2 CAS No.9000-91-3 Top Source Inquiry (/order.html?pid=14053&name=β-amylase) CatalogNATE-0762 ProductNameNative Sweet potato β-Amylase (/product/native-sweet-potato-amylase_1066.html) EC No.EC 3.2.1.2 CAS No.9000-91-3 SourceSweet potato Inquiry (/order.html?pid=1066&name=Native Sweet potato β-Amylase) CatalogNATE-0761 ProductNameNative Barley β-Amylase (/product/native-barley-amylase_1065.html) EC No.EC 3.2.1.2 https://www.creative-enzymes.com/similar/-Amylase_754.html 2/6

3. 2020/12/29 β-Amylase - Creative Enzymes CAS No.9000-91-3 SourceBarley Inquiry (/order.html?pid=1065&name=Native Barley β-Amylase) Related Protocols   (/pdf/EC%203.2.1.23β-GALACTOSIDASE.pdf)β-GALACTOSIDASE (/pdf/EC%203.2.1.23β- GALACTOSIDASE.pdf) -Enzymatic Assay Protocol Related Reading β-Amylase β-amylase (β-amylase), also known as starch β-1,4-maltosidase, is one of amylases, widely present in barley, wheat, sweet potato, soybean and other higher plants and Bacillus Microbes. It is the main saccharification agent for beer brewing and caramel (malt syrup) manufacture. High-malt syrups with a maltose content of 60% to 70% can be produced by saccharifying starch materials that have been acidified or liquefied with α-amylase produced by microorganisms such as Bacillus polymyxa and Bacillus megaterium. Services Top Figure 1. Protein structure of β-Amylase. https://www.creative-enzymes.com/similar/-Amylase_754.html 3/6

4. 2020/12/29 Source of β-Amylase β-Amylase - Creative Enzymes β-amylase is mainly found in higher plants, especially grains, such as barley, wheat, etc., also exists in sweet potatoes, soybeans, but not in animals. The β-amylase currently used in industry mainly includes plant β-amylase and microbial β-amylase.Due to the high production cost of plant-derived β-amylase, people have begun to attach importance to microbial-derived β-amylase. Since the 1960s, it has been found that Bacillus megaterium and Polymyxa. Bacteria and Bacillus cereus β- amylase, but their heat resistance is lower than plant β-amylase. Properties The active center of β-amylase contains a sulfhydryl group (-SH), therefore, some oxidants, heavy metal ions and sulfhydryl reagents can inactivate it, while reducing glutathione and cysteine have a protective effect. The optimal pH range of β-amylase and α-amylase are basically the same, generally around 5.0 ~ 6.5, but the stability of β-amylase is significantly lower than that of α- amylase, and generally will be inactivated above 70 ℃. The stability of β-amylase from different sources also varies greatly. The optimum temperature for soybean β-amylase is about 60 ℃, the optimum temperature for barley β-amylase is 50 ~ 55 ℃, and the most suitable for bacterial β- amylase. Suitable temperature is generally lower than 50 ℃. Functions β-amylase is an exo-amylase, which cuts the separated α-1,4 bonds from non-reducing ends when acting on starch, and the hydrolysate is all maltose. Because the amylase changes the configuration of C1 in the hydrolysate maltose molecule from alpha type to beta type during the hydrolysis process, it is called beta-amylase. β-amylase cannot hydrolyze the α-1,6 bond of amylopectin, nor can it continue to hydrolyze across branch points, so hydrolysis of amylopectin is incomplete, leaving large-molecule β-limit dextrins. When β-amylase hydrolyzes amylose, if the starch molecule consists of an even number of glucose units, the final hydrolysate is all maltose; if the starch molecule consists of an odd number of glucose units, the final hydrolysate contains a small amount of glucose in addition to maltose. When β-amylase hydrolyzes starch, since there are always macromolecules starting from the end of the molecule, the viscosity decreases slowly and cannot be used as a liquefaction enzyme, while β-amylase hydrolyzes starch hydrolysates such as maltodextrin and malto-oligosaccharide At the time, the hydrolysis rate is very fast, so it is used as a saccharification enzyme. Services Top Acute pancreatitis Increased serum amylase is more common in acute pancreatitis, it is one of the important diagnostic indicators of acute pancreatitis. Activity begins to increase 6 to 12 hours after onset, peaks at 24 hours, begins to decrease at 48 hours, and returns to normal after 3 to 5 days. Although the degree of increase in amylase activity is not necessarily related to the degree of pancreatic injury, the greater the degree of increase, the greater the possibility of acute pancreatitis, so although amylase is still used as a diagnosis of acute pancreatitis The index is preferred, but its specificity and sensitivity are not high enough. When acute pancreatitis is suspected, the patient's serum and urine https://www.creative-enzymes.com/similar/-Amylase_754.html 4/6

5. 2020/12/29 amylase activities should be continuously and dynamically observed, and combined with clinical conditions and other tests, such as pancreatic lipase, trypsin and other measurements, to make a diagnosis. β-Amylase - Creative Enzymes Figure 2. Acute pancreatitis. Services Top Sitemap () | Privacy Policy () | Terms and Conditions (/service/TERMS-AND-CONDITIONS-FOR- PROVISION-OF-PRODUCTS_406.html) Copyright ©2011 - 2020 Creative Enzymes. Contact Us   45-1 Ramsey Road, Shirley, NY 11967, USA  Email: info@creative-enzymes.com  Tel: 1-631-562-8517 1-516-512-3133  Fax: 1-631-938-8127 Distributors    To view the contact information for a specific location, select the desired country or region: country or region https://www.creative-enzymes.com/similar/-Amylase_754.html 5/6

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