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tBRG1

A. S. S. S. H P. S. H. H. K. H. P. *. tBRG1. . H. K. SII. X. S. S. *. X. B. NEO. tBRG1 bromo. C. X,K. P. S. EE S. H. *. M. 3.8 kb. tBRG1 bromo cDNA. tBRG1 bromop. 1073 AA. 127kD. Cu428 tBRG1 bromo. B. D.

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tBRG1

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  1. A S S S H P S H H K H P * tBRG1  H K SII X S S * X B NEO tBRG1bromo C X,K P S EE S H * M 3.8 kb tBRG1bromo cDNA tBRG1bromop 1073 AA 127kD Cu428 tBRG1bromo B D S SX 1(+P) 1(-P) 2(+P) 2(-P) tBrg1bromo1 x tBrg1bromo2 48/78 clones survived pm-r, mp-s: 25/48 pm-s, mp-r: 17/48 Pm-s, mp-s: 6/17 mp-s segregates 2.83:1 6.0kb 5.0kb tBRG1 (5.1kb) tBRG1bromo (3.5kb) 3.0kb 2.0kb X B2086 Cu428 C Mpr+ Mpr Mpr+ Mpr E tBrg1 bromop (pm-s, mp-s, II) (pm-s, mp-s, VI) Exconjugants Mpr+ Mpr 1 2 1 2 3 4 5 Biolistic transformation tBRGbromo construct, Select for pm-r 220 kDa (pm-r, mp-r, variable mating type) 97 kDa Lose mp-r by phenotypic assortment Mpr+ Mpr 66 kDa 30 kDa (pm-r, mp-s, variable mating type) Exconjugant phenotype 1: pm-r, mp-s 2,3 : pm-s, mp-r 4,5 : pm-s mp-s Mate pm-r, mp-s cells with each other Pick mating pairs into SPP Screen for pm-s, Mendelian assortment of mp-r

  2. DAPI FITC F MAC MAC G MAC MAC H MIC AN AN OM

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