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Richard Owen, 1843 defined concept of homologous organs

Richard Owen, 1843 defined concept of homologous organs Homeotic transformations of organs in plants… “Metamorphosis of Plants” Published by Johan Goethe, 1790.

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Richard Owen, 1843 defined concept of homologous organs

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  1. Richard Owen, 1843 defined concept of homologous organs Homeotic transformations of organs in plants… “Metamorphosis of Plants” Published by Johan Goethe, 1790

  2. Wild type. APETALA1:    PISTILLATA :   AGAMOUS:"A" class           "B" class          "C" class

  3. Wild type. APETALA1:    PISTILLATA :   AGAMOUS:"A" class           "B" class          "C" class

  4. AP1 mRNA AG1 mRNA AP1 in AG1 mutant

  5. ~550 MYA

  6. plena Shp1/2 Plena clade Ag Far AG clade

  7. Central zone adaxial abaxial apical basal phyllotaxy

  8. Abaxial domain Adaxial domain

  9. adaxial abaxial Lateral primordia formation/polarity: abaxial identity: crabsclaw, YABBY, KANADI - arabidopsis adaxial identity: phantastica - Antirrhinum roughsheath - maize phabulosa - arabidopsis

  10. McConnell and Barton. 1998. Development 125:2935 1. Ad/ab polarity is needed for lateral expansion 2. Adaxial cells determine initiation of the axial meristem Wild type Phb

  11. phabulosa Wild Type

  12. Dominant Phabulosa/Phavulota mutations HomeoBox- Zipper DNA binding domain START Sterol binding domain

  13. WT Phb

  14. Triple knockout phenotype: Emery et al. 2003. Current Biology, Vol. 13, 1768–1774, Dominant Phb phenotype

  15. Boundary required for Later growth of leaf blade Abaxial domain Adaxial domain

  16. Abaxial determinants: Development 126, 4117-4128 (1999) Members of the YABBYgene family specify abaxial cell fate in Arabidopsis Kellee R. Siegfried1,*, Yuval Eshed1, Stuart F. Baum1, Denichiro Otsuga2, Gary N. Drews2 and John L. Bowman1,‡ KANADI regulates organ polarity in Arabidopsis Randall A. Kerstetter, Krista Bollman, R. Alexandra Taylor, Kirsten Bomblies & R. Scott Poethig 2001 Nature 411:706-8

  17. Dominant Phabulosa/Phavulota mutations HomeoBox- Zipper DNA binding domain START Sterol binding domain

  18. Carrington and Ambros 2003, Science 301:337

  19. PTGS pathway in eukaryotes Carpel Factory (DCL1) Adapted from Williams and Rubin 2001, PNAS

  20. Jacobsen et al. 1998. Development 126:5231

  21. Kidner and Martienssen, Nature. 2004 Mar 4;428(6978):81-4 Argonaut phenotypes AGO is a component of the RISC RNA induced silencing complex

  22. PHB WT miR165 WT miR165 ago PHB ago

  23. Boundary required for Later growth of leaf blade Abaxial domain Adaxial domain

  24. Asymmetric expression of YAB3 requires KANADI function

  25. PHABULOSA expression in the radialized (adaxialized) KANADI triple mutant

  26. In tomato, YABBY is restricted to cytoplasmic dense cells at the margins of the ad/ab boundary

  27. Emery et al. 2003. Current Biology, Vol. 13, 1768–1774,

  28. Bohmert et al EMBO J 17:170-180

  29. Meristem initiation/maintenance: Knotted -maize shoot apical meristemless -arabidopsis Meristem size: clavata1 clavata2 clavata3 Lateral organ polarity: Abaxial genes: crabsclaw, YABBY Kanadi Adaxial genes: phabulosa Phavoluta Revoluta Organ number and polarity: Carpel Factory Argonaute

  30. Dominant Phabulosa/Phavulota mutations HomeoBox- Zipper DNA binding domain START Sterol binding domain

  31. Bohmert et al EMBO J 17:170-180

  32. adaxial abaxial Lateral primordia formation/polarity: abaxial identity: crabsclaw, YABBY, KANADI - arabidopsis adaxial identity: phantastica - Antirrhinum roughsheath - maize phabulosa - arabidopsis

  33. Carrington and Ambros 2003, Science 301:337

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