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Instrumentation

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Instrumentation

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    1. Instrumentation June 23, 2004

    2. outline Light Sources (UV/Vis) Wavelength selector (monochromator) Sample holder Detector Signal processor

    3. Outline

    4. Light Source

    5. Uv/vis lamp

    6. Visible lamp

    7. Visible lamp

    8. Irradiation of lamp

    9. Irradiation of lamp (xenon)

    10. Wavelength selector

    11. prism

    12. Grating

    13. Sample compartment (Sample holder)

    14. Cuvette

    15. detector

    16. Photo-multiplier tube

    17. Signal processor

    18. Types of Spectrophotometer

    19. Application of UV/vis spectrophotometer

    20. Structure elucidation

    21. Identification

    22. Purity

    23. Quantitative analysis

    24. Practical Applications of UV/vis Pharmacy Practice Ultraquin (psoriasis med. Needs UV. Act.) Pregnancy tests (colorimetric assays) Blood glucose tests, Bilichek ELISA’s Pharmaceutics pH titrations, purity measurement concentration measurement

    25. pKa Measurement with UV

    26. Practical Applications of Uv/vis Medicinal Chemistry compound ID (steroids, nucleosides) monitoring isomerization, chirality Pharmaceutical Biotechnology concentration/purity measurements monitoring conformation of protein drugs Pharmacokinetics/Med. Chem. HPLC monitoring and purification

    27. Pharmaceutical Apps. Of uv/vis On Line Analysis of Vitamin A and Coloring Dyes for the Pharmaceutical Industry Determination of Urinary Total Protein Output Analysis of total barbiturates Comparison of two physical light blocking agents for sunscreen lotions

    28. Determination of acetylsalicylic acid in aspirin using Total Fluorescence Spectroscopy Automated determination of the uniformity of dosage in Quinine Sulfate tablets using a Fibre Optics Autosampler Determining Cytochrome P450 by UV-Vis Spectrophotometry Light Transmittance of Plastic Pharmaceutical Containers Pharmaceutical Apps. Of uv/vis

    29. TIME IS PRECIOUS

    30. TIME IS PRECIOUS To realise the value of 1 minute, ask a person who has missed the train To realise the value of 1 second, ask a person who has survived an accident To realise the value of 1 milli second, ask the person who has won a silver medal in Olympics.

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